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Characterization of Toll-like receptors 1-10 in spotted hyenas

Citation

Flies, AS and Maksimoski, MT and Mansfield, LS and Weldele, ML and Holekamp, KE, Characterization of Toll-like receptors 1-10 in spotted hyenas, Veterinary Research Communications: An International Journal Publishing Topical Reviews and Research Articles on All Aspects of The Veterinary Sciences, 38, (2) pp. 165-70. ISSN 0165-7380 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 Springer Science+Business Media Dordrecht

DOI: doi:10.1007/s11259-014-9592-3

Abstract

Previous research has shown that spotted hyenas (Crocuta crocuta) regularly survive exposure to deadly pathogens such as rabies, canine distemper virus, and anthrax, suggesting that they have robust immune defenses. Toll-like receptors (TLRs) recognize conserved molecular patterns and initiate a wide range of innate and adaptive immune responses. TLR genes are evolutionarily conserved, and assessing TLR expression in various tissues can provide insight into overall immunological organization and function. Studies of the hyena immune system have been minimal thus far due to the logistical and ethical challenges of sampling and preserving the immunological tissues of this and other long-lived, wild species. Tissue samples were opportunistically collected from captive hyenas humanely euthanized for a separate study. We developed primers to amplify partial sequences for TLRs 1-10, sequenced the amplicons, compared sequence identity to those in other mammals, and quantified TLR expression in lymph nodes, spleens, lungs, and pancreases. Results show that hyena TLR DNA and protein sequences are similar to TLRs in other mammals, and that TLRs 1-10 were expressed in all tissues tested. This information will be useful in the development of new assays to understand the interactions among the hyena immune system, pathogens, and the microbial communities that inhabit hyenas.

Item Details

Item Type:Refereed Article
Keywords:toll-like receptor, TLR
Research Division:Medical and Health Sciences
Research Group:Immunology
Research Field:Cellular Immunology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Immune System and Allergy
Author:Flies, AS (Dr Andy Flies)
ID Code:102803
Year Published:2014
Web of Science® Times Cited:4
Deposited By:Menzies Institute for Medical Research
Deposited On:2015-09-07
Last Modified:2017-11-07
Downloads:0

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