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Residual soil DNA extraction increases the discriminatory power between samples

Citation

Young, JM and Weyrich, LS and Clarke, LJ and Cooper, A, Residual soil DNA extraction increases the discriminatory power between samples, Forensic Science, Medicine, and Pathology, 11, (2) pp. 268-272. ISSN 1547-769X (2015) [Refereed Article]

Copyright Statement

Copyright 2015 Springer Science+Business Media

DOI: doi:10.1007/s12024-015-9662-z

Abstract

Forensic soil analysis relies on capturing an accurate and reproducible representation of the diversity from limited quantities of soil; however, inefficient DNA extraction can markedly alter the taxonomic abundance. The performance of a standard commercial DNA extraction kit (MOBIO PowerSoil DNA Isolation kit) and three modified protocols of this kit: soil pellet re-extraction (RE); an additional 24-h lysis incubation step at room temperature (RT); and 24-h lysis incubation step at 55C (55) were compared using high-throughput sequencing of the internal transcribed spacer I ribosomal DNA. DNA yield was not correlated with fungal diversity and the four DNA extraction methods displayed distinct fungal community profiles for individual samples, with some phyla detected exclusively using the modified methods. Application of a 24h lysis step will provide a more complete inventory of fungal biodiversity, and re-extraction of the residual soil pellet offers a novel tool for increasing discriminatory power between forensic soil samples.

Item Details

Item Type:Refereed Article
Keywords:soil, forensics, metagenomics, DNA extraction, high-throughput sequencing
Research Division:Biological Sciences
Research Group:Other Biological Sciences
Research Field:Forensic Biology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Clarke, LJ (Dr Laurence Clarke)
ID Code:102566
Year Published:2015
Web of Science® Times Cited:5
Deposited By:CRC-Antarctic Climate & Ecosystems
Deposited On:2015-08-28
Last Modified:2017-11-03
Downloads:0

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