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Transforming growth factor alpha increases extracellular matrix within a transgenic mouse model of airway disease


Elliot, J and Wang, K and Le Cras, T and Larcombe, A and Zosky, GR and James, A and Noble, P, Transforming growth factor alpha increases extracellular matrix within a transgenic mouse model of airway disease, Respirology, 27 March - 1 April, 2015, Gold Coast, Australia, pp. 96. ISSN 1323-7799 (2015) [Conference Extract]

DOI: doi:10.1111/resp.12495


Increased expression of transforming growth factor alpha (TGF-α) and its receptor, epidermal growth factor receptor, may contribute to the increased thickness of airway smooth muscle (ASM) layer in asthma.

Aim: In a transgenic mouse model of increased TGF-α expression, we have examined the number and size of ASM cells and the volume fraction of extracellular matrix (ECM) within the ASM layer.

Methods: TGF-α was conditionally expressed in the airway epithelium of transgenic mice by exposure to doxycycline in chow (from 0, 10 and 14 days) which induces deficiency in early growth response-1 which upregulates TGF-α induced airway disease. On formalin fixed transverse airway sections, stained with haematoxylin (30 μm), the volume density (NV) of ASM cell nuclei was estimated using an optical disector (◊1000) and the ASM area measured. On contiguous thin (0.5 μm) sections (Massonís trichrome) the volume fraction of ECM (VvECM) was estimated. The mean ASM cell volume (VC = 1/ (NV ◊ VvASM)), the number of ASM cells (NL = NV ◊ ASM area ◊ 1 mm) and volumes of ASM and ECM per mm of airway were calculated.

Conclusion: The volume fraction of ECM in the layer of smooth muscle and the average ASM cell size (hypertrophy) are increased in this mouse model, although considerable variablility exists between animals.

Item Details

Item Type:Conference Extract
Research Division:Biomedical and Clinical Sciences
Research Group:Cardiovascular medicine and haematology
Research Field:Respiratory diseases
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Zosky, GR (Professor Graeme Zosky)
ID Code:102388
Year Published:2015
Deposited By:Medicine
Deposited On:2015-08-18
Last Modified:2016-03-22

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