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Intrinsic Differences Of Epithelial Tight Junctions In Asthmatic Airway Epithelium And Barrier Compromisation Following Human Rhinoviral Insult
Introduction: Junctional protein complexes such as tight junctions (TJ) provide a physical barrier against external insults. Although various aspects of TJs have been extensively assessed, few studies have directly addressed whether these are intrinsically different in asthmatic epithelium and the consequence on barrier function of disassembly post- human rhinovirus (HRV) infection. This study aimed to assess basal TJ expression and barrier function prior to and post-HRV infection in both monolayer and air-liquid interface (ALI) cultures of healthy and asthmatic epithelium.
Methods: Primary airway epithelial cells from healthy and asthmatic children obtained via bronchial brushings and cultured as previously described (Kicic et al. 2006) were also differentiated into ALI and subsequently infected with HRV-1B over 24h. Tight junction gene expression was assessed via qPCR, barrier integrity measured by TJ protein expression of claudin-1, occludin and zonula occluden-1 (ZO-1) via immunocytochemistry (ICC), in-cell western (ICW) and confocal microscopy while barrier function was assessed via transepithelial electrical resistance (R ) measurement and a permeability assay.
Results: Ex vivo TJ gene expression was significantly increased in asthmatic epithelium for claudin-1 and occludin, while ZO-1 was not significantly different when compared to healthy epithelium. In contrast, protein expression for claudin-1, occludin and ZO-1 observed via ICC was markedly reduced in asthmatics compared to healthy controls. Semi-quantitative assessment of claudin-1, occludin and ZO-1 protein expression via ICW corroborated ICC findings and demonstrated significantly reduced basal membrane TJ expression in asthmatics compared to healthy controls (p<0.05). A greater effect on TJ disassembly was observed within the asthmatic epithelium post infection which was concurrent with a marked increase in transepithelial permeability. Semi-quantitative assessment of occludin and ZO-1 protein expression via confocal microscopy corroborated ICW findings and demonstrated reduced basal membrane TJ expression in asthmatic compared to healthy controls. A greater effect on TJ disassembly was observed within the ALI asthmatic epithelium post-infection and this was concurrent with a significant decrease in R and a marked increase in transepithelial permeability.
Conclusion: This study demonstrates significant differences in basal membrane TJ protein expression between healthy and asthmatic epithelial cells, suggesting intrinsic differences between healthy and mild-asthmatic epithelium. Furthermore, post HRV infection, an exaggerated disassembly in the asthmatic epithelial cells, concomitant with increased transepithelial permeability suggests elevated trafficking of small sized aeroallergens into the sub-epithelial space, contributing to asthma exacerbations.
History
Publication title
American Journal of Respiratory and Critical Care MedicineISSN
1073-449XDepartment/School
Tasmanian School of MedicinePublisher
American Thoracic SocietyPlace of publication
United StatesEvent title
American Thoracic Society 2015 International ConferenceEvent Venue
Denver, ColoradoDate of Event (Start Date)
2015-05-15Date of Event (End Date)
2015-05-20Repository Status
- Restricted