Looi, K and Buckley, A and Rigby, P and Garrett, LW and Iosifidis, T and Lannigan, F and Knight, DA and Zosky, GR and Larcombe, A and Ling, K and Martinovich, KM and Kicic-Starcevich, E and Shaw, NC and Sutanto, EN and Kicic, A and Stick, S, Intrinsic Differences Of Epithelial Tight Junctions In Asthmatic Airway Epithelium And Barrier Compromisation Following Human Rhinoviral Insult, American Journal of Respiratory and Critical Care Medicine, 15-20 May, 2015, Denver, Colorado ISSN 1073-449X (2015) [Conference Extract]
Introduction: Junctional protein complexes such as tight junctions (TJ) provide a physical barrier against external insults. Although various aspects of TJs have been extensively assessed, few studies have directly addressed whether these are intrinsically different in asthmatic epithelium and the consequence on barrier function of disassembly post- human rhinovirus (HRV) infection. This study aimed to assess basal TJ expression and barrier function prior to and post-HRV infection in both monolayer and air-liquid interface (ALI) cultures of healthy and asthmatic epithelium.
Methods: Primary airway epithelial cells from healthy and asthmatic children obtained via bronchial brushings and cultured as previously described (Kicic et al. 2006) were also differentiated into ALI and subsequently infected with HRV-1B over 24h. Tight junction gene expression was assessed via qPCR, barrier integrity measured by TJ protein expression of claudin-1, occludin and zonula occluden-1 (ZO-1) via immunocytochemistry (ICC), in-cell western (ICW) and confocal microscopy while barrier function was assessed via transepithelial electrical resistance (R ) measurement and a permeability assay.
Results: Ex vivo TJ gene expression was significantly increased in asthmatic epithelium for claudin-1 and occludin, while ZO-1 was not significantly different when compared to healthy epithelium. In contrast, protein expression for claudin-1, occludin and ZO-1 observed via ICC was markedly reduced in asthmatics compared to healthy controls. Semi-quantitative assessment of claudin-1, occludin and ZO-1 protein expression via ICW corroborated ICC findings and demonstrated significantly reduced basal membrane TJ expression in asthmatics compared to healthy controls (p<0.05). A greater effect on TJ disassembly was observed within the asthmatic epithelium post infection which was concurrent with a marked increase in transepithelial permeability. Semi-quantitative assessment of occludin and ZO-1 protein expression via confocal microscopy corroborated ICW findings and demonstrated reduced basal membrane TJ expression in asthmatic compared to healthy controls. A greater effect on TJ disassembly was observed within the ALI asthmatic epithelium post-infection and this was concurrent with a significant decrease in R and a marked increase in transepithelial permeability.
Conclusion: This study demonstrates significant differences in basal membrane TJ protein expression between healthy and asthmatic epithelial cells, suggesting intrinsic differences between healthy and mild-asthmatic epithelium. Furthermore, post HRV infection, an exaggerated disassembly in the asthmatic epithelial cells, concomitant with increased transepithelial permeability suggests elevated trafficking of small sized aeroallergens into the sub-epithelial space, contributing to asthma exacerbations.
|Item Type:||Conference Extract|
|Keywords:||rhinovirus, epithelium, tight junctions|
|Research Division:||Medical and Health Sciences|
|Research Group:||Cardiorespiratory Medicine and Haematology|
|Research Field:||Respiratory Diseases|
|Objective Group:||Clinical Health (Organs, Diseases and Abnormal Conditions)|
|Objective Field:||Respiratory System and Diseases (incl. Asthma)|
|UTAS Author:||Zosky, GR (Professor Graeme Zosky)|
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