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Chloroplast-generated ROS dominate NaCl-induced K+ efflux in wheat leaf mesophyll

journal contribution
posted on 2023-05-18, 12:19 authored by Wu, H, Svetlana ShabalaSvetlana Shabala, Meixue ZhouMeixue Zhou, Sergey ShabalaSergey Shabala
Mesophyll K+ retention ability has been recently reported as an important component of salinity stress tolerance in wheat. In order to investigate the role of ROS in regulating NaCl-induced K+ efflux in wheat leaf mesophyll, a series of pharmacological experiments was conducted using MV (methyl viologen, superoxide radical inducer), DPI (an inhibitor of NADPH oxidase), H2O2 (to mimic apoplastic ROS), and EGCG ((−)-Epigallocatechin gallate, ROS scavenger). Mesophyll pre-treatment with 10 μM MV resulted in a significantly higher NaCl-induced K+ efflux in leaf mesophyll, while 50 μM EGCG pre-treatment alleviated K+ leakage under salt stress. No significant change in NaCl-induced K+ efflux in leaf mesophyll was found in specimens pre-treated by H2O2 and DPI, compared with the control. The highest NaCl-induced H+ efflux in leaf mesophyll was also found in samples pre-treated with MV, suggesting a futile cycle between increased H+-ATPase activity and ROS-induced K+ leak. Overall, it is suggested that, under saline stress, K+ efflux from wheat mesophyll is mediated predominantly by non-selective cation channels (NSCC) regulated by ROS produced in chloroplasts, at least in bread wheat.

Funding

Grains Research & Development Corporation

History

Publication title

Plant Signalling and Behavior

Volume

10

Issue

5

Article number

e1013793

Number

e1013793

Pagination

1-4

ISSN

1559-2316

Department/School

Tasmanian Institute of Agriculture (TIA)

Publisher

Landes Bioscience

Place of publication

United States of America

Rights statement

© 2015 Taylor & Francis Group, LLC

Repository Status

  • Restricted

Socio-economic Objectives

Barley

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