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Chloroplast-generated ROS dominate NaCl-induced K+ efflux in wheat leaf mesophyll


Wu, H and Shabala, L and Zhou, M and Shabala, S, Chloroplast-generated ROS dominate NaCl-induced K+ efflux in wheat leaf mesophyll, Plant Signalling and Behavior, 10, (5) Article e1013793. ISSN 1559-2316 (2015) [Refereed Article]

Copyright Statement

2015 Taylor & Francis Group, LLC

DOI: doi:10.1080/15592324.2015.1013793


Mesophyll K+ retention ability has been recently reported as an important component of salinity stress tolerance in wheat. In order to investigate the role of ROS in regulating NaCl-induced K+ efflux in wheat leaf mesophyll, a series of pharmacological experiments was conducted using MV (methyl viologen, superoxide radical inducer), DPI (an inhibitor of NADPH oxidase), H2O2 (to mimic apoplastic ROS), and EGCG ((−)-Epigallocatechin gallate, ROS scavenger). Mesophyll pre-treatment with 10μM MV resulted in a significantly higher NaCl-induced K+ efflux in leaf mesophyll, while 50μM EGCG pre-treatment alleviated K+ leakage under salt stress. No significant change in NaCl-induced K+ efflux in leaf mesophyll was found in specimens pre-treated by H2O2 and DPI, compared with the control. The highest NaCl-induced H+ efflux in leaf mesophyll was also found in samples pre-treated with MV, suggesting a futile cycle between increased H+-ATPase activity and ROS-induced K+ leak. Overall, it is suggested that, under saline stress, K+ efflux from wheat mesophyll is mediated predominantly by non-selective cation channels (NSCC) regulated by ROS produced in chloroplasts, at least in bread wheat.

Item Details

Item Type:Refereed Article
Keywords:barley, salinity
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Crop and pasture production
Research Field:Crop and pasture improvement (incl. selection and breeding)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Grains and seeds
Objective Field:Barley
UTAS Author:Wu, H (Mr Honghong Wu)
UTAS Author:Shabala, L (Associate Professor Lana Shabala)
UTAS Author:Zhou, M (Professor Meixue Zhou)
UTAS Author:Shabala, S (Professor Sergey Shabala)
ID Code:102333
Year Published:2015
Web of Science® Times Cited:25
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2015-08-13
Last Modified:2017-11-06

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