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Attached and planktonic Listeria monocytogenes global proteomic responses and associated influence of strain genetics and temperature

Citation

Mata, MM and da Silva, WP and Wilson, R and Lowe, E and Bowman, JP, Attached and planktonic Listeria monocytogenes global proteomic responses and associated influence of strain genetics and temperature, Journal of Proteome Research, 14, (2) pp. 1161-1173. ISSN 1535-3893 (2015) [Refereed Article]

Copyright Statement

2014 American Chemical Society

DOI: doi:10.1021/pr501114e

Abstract

Contamination of industrial and domestic food usage environments by the attachement of bacterial food-borne pathogen Listeria monocytogenes has public health and economic implications. Comprehensive proteomics experiments using label-free liquid chromatography/tandem mass spectrometry were used to compare the proteomes of two different L. monocytogenes strains (Siliken_1/2c and F2365_4b), which show very different capacities to attach to surfaces. Growth temperature and strain type were highly influential on the proteomes in both attached and planktonic cells. On the basis of the proteomic data, it is highly unlikely that specific surface proteins play a direct role in adherence to inanimate surfaces. Instead, strain-dependent responses related to cell envelope polymer biosynthesis and stress response regulation likely contribute to a different ability to attach and also to survive external stressors. Collectively, the divergent proteome-level responses observed define strain- and growth-temperature-dependent differences relevant to attachment efficacy, highlight relevant proteins involved in stress protection in attached cells, and suggest that strain differences and growth conditions are important in relation to environmental persistence.

Item Details

Item Type:Refereed Article
Keywords:Listeria, biofilms, proteomics, Listeria monocytogenes, attachment, persistence, temperature
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Bacteriology
Objective Division:Health
Objective Group:Public Health (excl. Specific Population Health)
Objective Field:Food Safety
Author:Wilson, R (Dr Richard Wilson)
Author:Bowman, JP (Associate Professor John Bowman)
ID Code:102278
Year Published:2015
Web of Science® Times Cited:2
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2015-08-10
Last Modified:2017-11-06
Downloads:0

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