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Oxygen deficiency and salinity affect cell-specific ion concentrations in adventitious roots of barley (Hordeum vulgare)


Kotula, L and Clode, PL and Striker, GG and Pedersen, O and Lauchli, A and Shabala, S and Colmer, TD, Oxygen deficiency and salinity affect cell-specific ion concentrations in adventitious roots of barley (Hordeum vulgare), New Phytologist, 208, (4) pp. 1114-1125. ISSN 0028-646X (2015) [Refereed Article]

Copyright Statement

Copyright 2015 The Authors. New Phytologist © 2015 New Phytologist Trust

DOI: doi:10.1111/nph.13535


  • Oxygen deficiency associated with soil waterlogging adversely impacts root respiration and nutrient acquisition. We investigated the effects of O2 deficiency and salinity (100 mM NaCl) on radial O2 concentrations and cell-specific ion distributions in adventitious roots of barley (Hordeum vulgare).
  • Microelectrode profiling measured O2 concentrations across roots in aerated, aerated saline, stagnant or stagnant saline media. X-ray microanalysis at two positions behind the apex determined the cell-specific elemental concentrations of potassium (K), sodium (Na) and chloride (Cl) across roots.
  • Severe O2 deficiency occurred in the stele and apical regions of roots in stagnant solutions. O2 deficiency in the stele reduced the concentrations of K, Na and Cl in the pericycle and xylem parenchyma cells at the subapical region. Near the root apex, Na declined across the cortex in roots from the aerated saline solution but was relatively high in all cell types in roots from the stagnant saline solution.
  • Oxygen deficiency has a substantial impact on cellular ion concentrations in roots. Both pericycle and xylem parenchyma cells are involved in energy-dependent K loading into the xylem and in controlling radial Na and Cl transport. At root tips, accumulation of Na in the outer cell layers likely contributed to reduction of Na in inner cells of the tips.
  • Item Details

    Item Type:Refereed Article
    Keywords:hypoxia, waterlogging, flooding, oxygen, salinity, barley, Hordeum vulgare, O2 deficiency, potassium, root aeration, salinity x waterlogging interaction, sodium, stele hypoxia, X-ray microanalysis
    Research Division:Biological Sciences
    Research Group:Plant biology
    Research Field:Plant physiology
    Objective Division:Plant Production and Plant Primary Products
    Objective Group:Grains and seeds
    Objective Field:Barley
    UTAS Author:Shabala, S (Professor Sergey Shabala)
    ID Code:102231
    Year Published:2015
    Web of Science® Times Cited:46
    Deposited By:Tasmanian Institute of Agriculture
    Deposited On:2015-08-05
    Last Modified:2017-11-02

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