Mercaptopropylsilica and silver nanoparticle (AgNP)-coated mercaptopropylsilica (Nucleosil 300, particle size 5 m and pore size 10 nm) were prepared and their suitability as stationary phases for reversed-phase high-performance liquid chromatography (RP-HPLC) was evaluated. The retention mechanism was investigated for both ionogenic and non-ionogenic compounds using 150 x 4.6 mm i.d. columns, with emphasis on non-specific hydrophobic interactions and the possibility of specific hydrogen-bonding and donor-acceptor interactions between solutes and stationary phases. The retention of more than 40 substances including pyridines, anilines, phenols, benzoic acids and others was investigated. A different selectivity was obtained in the case of a series of anilines and pyridines. However, both phases showed a non-orthogonal retention mechanism, the latter being related to electron donor-acceptor interactions. The presence of hydrophilic interaction liquid chromatography (HILIC) retention behavior was demonstrated for mercaptopropylsilica phase.

Item Type:	Refereed Article
Keywords:	coordination, hydrogen bonding, hydrophilic interaction liquid chromatography, mercaptopropylsilica, reversedphase high performance liquid chromatography, silver nanoparticles, stationary phase
Research Division:	Chemical Sciences
Research Group:	Analytical chemistry
Research Field:	Separation science
Objective Division:	Expanding Knowledge
Objective Group:	Expanding knowledge
Objective Field:	Expanding knowledge in the chemical sciences
UTAS Author:	Sandron, S (Dr Sara Sandron)
UTAS Author:	Paull, B (Professor Brett Paull)
UTAS Author:	Nesterenko, PN (Professor Pavel Nesterenko)
ID Code:	102221
Year Published:	2015
Funding Support:	Australian Research Council (DP130101518)
Deposited By:	Chemistry
Deposited On:	2015-08-05
Last Modified:	2016-05-17
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