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Mass spectrometric determination of n-hydroxyphenacetin in urine using multiple metastable peak monitoring following thin-layer chromatography

Citation

Davies, NW and Veronese, ME and McLean, S, Mass spectrometric determination of n-hydroxyphenacetin in urine using multiple metastable peak monitoring following thin-layer chromatography, Journal of Chromatography B: Biomedical Sciences and Applications, 310 pp. 179-187. ISSN 0378-4347 (1984) [Refereed Article]

Copyright Statement

Copyright 1984 Elsevier Science Publishers B.V.

DOI: doi:10.1016/0378-4347(84)80079-1

Abstract

This work describes a method for the quantitative determination of the labile, toxic N-hydroxy metabolite of phenacetin in urine. A thin-layer chromatography step was used for the preliminary purification of extracts, and the specificity of the assay was based on the monitoring of specific metastable decompositions in a forward geometry double-focussing mass spectrometer, in a manner analogous to conventional tandem mass spectrometry. This precluded the need for a gas chromatographic separation, thus minimizing thermal decomposition which can occur with these compounds, as well as enabling very rapid analyses.

Item Details

Item Type:Refereed Article
Keywords:chromatography, thin layer, drug stability, human, methylation, phenacetin, spectrum analysis, mass
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Davies, NW (Associate Professor Noel Davies)
Author:Veronese, ME (Mr Maurice Veronese)
Author:McLean, S (Professor Stuart McLean)
ID Code:102202
Year Published:1984
Web of Science® Times Cited:12
Deposited By:Central Science Laboratory
Deposited On:2015-08-04
Last Modified:2015-09-07
Downloads:0

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