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Identification and quantification of endogenous gibberellins in apical buds and the cambial region of Eucalyptus
Citation
Hasan, O and Ridoutt, BG and Ross, JJ and Davies, NW and Reid, JB, Identification and quantification of endogenous gibberellins in apical buds and the cambial region of Eucalyptus, Physiologia Plantarum, 90, (3) pp. 475-480. ISSN 0031-9317 (1994) [Refereed Article]
Copyright Statement
Copyright 1994 Physiologia Plantarum
DOI: doi:10.1111/j.1399-3054.1994.tb08804.x
Abstract
Endogenous gibberellins (GAs) were extracted and purified from apical buds of Eucalyptus nitens (Deane and Maid.) Maid. and the cambial region of E. globulus (Labill.), then analysed by capillary gas chromatography-mass spectrometry. GA1, GA19, GA20 and GA29 were identified by full scan mass spectra, Kovats retention indices and high resolution selected ion monitoring. Using deuterated internal standards, GA1, GA19, GA20 and putative GA29 and GA53 Were quantified in the apical buds, while GA4, GA8, GA9 and GA44 were shown to be either absent or present at very low levels. From the cambial region, GA1 and GA20 were quantified at levels of 0.30 ng (g fresh weight)-1 and 8.8 ng (g fresh weight)-1 respectively. These data suggest that the early 13-hydroxylation pathway is the dominant pathway for GA biosynthesis in Eucalyptus.
Item Details
Item Type: | Refereed Article |
---|---|
Research Division: | Biological Sciences |
Research Group: | Biochemistry and cell biology |
Research Field: | Signal transduction |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Hasan, O (Dr Omar Hasan) |
UTAS Author: | Ross, JJ (Associate Professor John Ross) |
UTAS Author: | Davies, NW (Associate Professor Noel Davies) |
UTAS Author: | Reid, JB (Professor Jim Reid) |
ID Code: | 102195 |
Year Published: | 1994 |
Web of Science® Times Cited: | 29 |
Deposited By: | Central Science Laboratory |
Deposited On: | 2015-08-04 |
Last Modified: | 2015-09-07 |
Downloads: | 0 |
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