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Identification and quantification of endogenous gibberellins in apical buds and the cambial region of Eucalyptus

Citation

Hasan, O and Ridoutt, BG and Ross, JJ and Davies, NW and Reid, JB, Identification and quantification of endogenous gibberellins in apical buds and the cambial region of Eucalyptus, Physiologia Plantarum, 90, (3) pp. 475-480. ISSN 0031-9317 (1994) [Refereed Article]

Copyright Statement

Copyright 1994 Physiologia Plantarum

DOI: doi:10.1111/j.1399-3054.1994.tb08804.x

Abstract

Endogenous gibberellins (GAs) were extracted and purified from apical buds of Eucalyptus nitens (Deane and Maid.) Maid. and the cambial region of E. globulus (Labill.), then analysed by capillary gas chromatography-mass spectrometry. GA1, GA19, GA20 and GA29 were identified by full scan mass spectra, Kovats retention indices and high resolution selected ion monitoring. Using deuterated internal standards, GA1, GA19, GA20 and putative GA29 and GA53 Were quantified in the apical buds, while GA4, GA8, GA9 and GA44 were shown to be either absent or present at very low levels. From the cambial region, GA1 and GA20 were quantified at levels of 0.30 ng (g fresh weight)-1 and 8.8 ng (g fresh weight)-1 respectively. These data suggest that the early 13-hydroxylation pathway is the dominant pathway for GA biosynthesis in Eucalyptus.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Signal Transduction
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Hasan, O (Dr Omar Hasan)
Author:Ross, JJ (Associate Professor John Ross)
Author:Davies, NW (Associate Professor Noel Davies)
Author:Reid, JB (Professor Jim Reid)
ID Code:102195
Year Published:1994
Web of Science® Times Cited:29
Deposited By:Central Science Laboratory
Deposited On:2015-08-04
Last Modified:2015-09-07
Downloads:0

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