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Enantiospecific gas chromatographic-mass spectrometric procedure for the determination of ketoprofen and ibuprofen in synovial fluid and plasma: application to protein binding studies
journal contribution
posted on 2023-05-18, 12:04 authored by Jack, DS, Rumble, RH, Noel DaviesNoel Davies, Francis, HWA method for the enantiospecific quantitation of two commonly prescribed non-steroidal anti-inflammatory drugs (ketoprofen and ibuprofen) is described. The method involves formation of a mixed anhydride of the drug with ethylchloroformate and subsequent conversion to an amide by reaction with optically active amphetamine. The subsequently formed diastereomers are separated by gas chromatography-mass spectrometry using selected-ion monitoring. The assay is capable of quantifying ketoprofen (2 ng/ml) and ibuprofen (3 ng/ml) enantiomers from a 200-μl sample of synovial fluid or plasma and is particularly suitable for protein binding studies.
History
Publication title
Journal of Chromatography B: Biomedical Sciences and ApplicationsVolume
584Pagination
189-197ISSN
0378-4347Department/School
School of Pharmacy and PharmacologyPublisher
Elsevier BVPlace of publication
NetherlandsRights statement
Copyright 1992 Elsevier Science Publishers B.V.Repository Status
- Restricted