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Enantiospecific gas chromatographic-mass spectrometric procedure for the determination of ketoprofen and ibuprofen in synovial fluid and plasma: application to protein binding studies
Citation
Jack, DS and Rumble, RH and Davies, NW and Francis, HW, Enantiospecific gas chromatographic-mass spectrometric procedure for the determination of ketoprofen and ibuprofen in synovial fluid and plasma: application to protein binding studies, Journal of Chromatography B: Biomedical Sciences and Applications, 584, (2) pp. 189-197. ISSN 0378-4347 (1992) [Refereed Article]
Copyright Statement
Copyright 1992 Elsevier Science Publishers B.V.
DOI: doi:10.1016/0378-4347(92)80575-B
Abstract
A method for the enantiospecific quantitation of two commonly prescribed non-steroidal anti-inflammatory drugs (ketoprofen and ibuprofen) is described. The method involves formation of a mixed anhydride of the drug with ethylchloroformate and subsequent conversion to an amide by reaction with optically active amphetamine. The subsequently formed diastereomers are separated by gas chromatography-mass spectrometry using selected-ion monitoring. The assay is capable of quantifying ketoprofen (2 ng/ml) and ibuprofen (3 ng/ml) enantiomers from a 200-μl sample of synovial fluid or plasma and is particularly suitable for protein binding studies.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Research Division: | Chemical Sciences |
| Research Group: | Analytical Chemistry |
| Research Field: | Separation Science |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding Knowledge |
| Objective Field: | Expanding Knowledge in the Chemical Sciences |
| Author: | Jack, DS (Mr David Jack) |
| Author: | Rumble, RH (Dr Roger Rumble) |
| Author: | Davies, NW (Associate Professor Noel Davies) |
| ID Code: | 102180 |
| Year Published: | 1992 |
| Web of Science® Times Cited: | 20 |
| Deposited By: | Central Science Laboratory |
| Deposited On: | 2015-08-03 |
| Last Modified: | 2015-09-22 |
| Downloads: | 0 |
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