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Enantiospecific gas chromatographic-mass spectrometric procedure for the determination of ketoprofen and ibuprofen in synovial fluid and plasma: application to protein binding studies

Citation

Jack, DS and Rumble, RH and Davies, NW and Francis, HW, Enantiospecific gas chromatographic-mass spectrometric procedure for the determination of ketoprofen and ibuprofen in synovial fluid and plasma: application to protein binding studies, Journal of Chromatography B: Biomedical Sciences and Applications, 584, (2) pp. 189-197. ISSN 0378-4347 (1992) [Refereed Article]

Copyright Statement

Copyright 1992 Elsevier Science Publishers B.V.

DOI: doi:10.1016/0378-4347(92)80575-B

Abstract

A method for the enantiospecific quantitation of two commonly prescribed non-steroidal anti-inflammatory drugs (ketoprofen and ibuprofen) is described. The method involves formation of a mixed anhydride of the drug with ethylchloroformate and subsequent conversion to an amide by reaction with optically active amphetamine. The subsequently formed diastereomers are separated by gas chromatography-mass spectrometry using selected-ion monitoring. The assay is capable of quantifying ketoprofen (2 ng/ml) and ibuprofen (3 ng/ml) enantiomers from a 200-μl sample of synovial fluid or plasma and is particularly suitable for protein binding studies.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Jack, DS (Mr David Jack)
Author:Rumble, RH (Dr Roger Rumble)
Author:Davies, NW (Associate Professor Noel Davies)
ID Code:102180
Year Published:1992
Web of Science® Times Cited:20
Deposited By:Central Science Laboratory
Deposited On:2015-08-03
Last Modified:2015-09-22
Downloads:0

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