eCite Digital Repository
Enantiospecific gas chromatographic-mass spectrometric procedure for the determination of ketoprofen and ibuprofen in synovial fluid and plasma: application to protein binding studies
Citation
Jack, DS and Rumble, RH and Davies, NW and Francis, HW, Enantiospecific gas chromatographic-mass spectrometric procedure for the determination of ketoprofen and ibuprofen in synovial fluid and plasma: application to protein binding studies, Journal of Chromatography B: Biomedical Sciences and Applications, 584, (2) pp. 189-197. ISSN 0378-4347 (1992) [Refereed Article]
Copyright Statement
Copyright 1992 Elsevier Science Publishers B.V.
DOI: doi:10.1016/0378-4347(92)80575-B
Abstract
A method for the enantiospecific quantitation of two commonly prescribed non-steroidal anti-inflammatory drugs (ketoprofen and ibuprofen) is described. The method involves formation of a mixed anhydride of the drug with ethylchloroformate and subsequent conversion to an amide by reaction with optically active amphetamine. The subsequently formed diastereomers are separated by gas chromatography-mass spectrometry using selected-ion monitoring. The assay is capable of quantifying ketoprofen (2 ng/ml) and ibuprofen (3 ng/ml) enantiomers from a 200-μl sample of synovial fluid or plasma and is particularly suitable for protein binding studies.
Item Details
Item Type: | Refereed Article |
---|---|
Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Separation science |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Jack, DS (Mr David Jack) |
UTAS Author: | Rumble, RH (Dr Roger Rumble) |
UTAS Author: | Davies, NW (Associate Professor Noel Davies) |
ID Code: | 102180 |
Year Published: | 1992 |
Web of Science® Times Cited: | 21 |
Deposited By: | Central Science Laboratory |
Deposited On: | 2015-08-03 |
Last Modified: | 2015-09-22 |
Downloads: | 0 |
Repository Staff Only: item control page