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Proteomic insight into functional changes of proteorhodopsin-containing bacterial species Psychroflexus torquis under different illumination and salinity levels

Citation

Feng, S and Powell, SM and Wilson, R and Bowman, JP, Proteomic insight into functional changes of proteorhodopsin-containing bacterial species Psychroflexus torquis under different illumination and salinity levels, Journal of Proteome Research, 14, (9) pp. 3848-3858. ISSN 1535-3893 (2015) [Refereed Article]

Copyright Statement

2015 American Chemical Society

DOI: doi:10.1021/acs.jproteome.5b00241

Abstract

The extremely psychrophilic proteorhodopsin-containing bacterial species Psychroflexus torquis is considered to be a model sea-ice microorganism, which has adapted to an epiphytic lifestyle. So far, not much is known about proteorhodopsin-based phototrophy and associated life strategies of sea ice bacteria, although it has been previously shown that P. torquis can gain growth advantage from light using a proteorhodopsin proton pump, the activity of which is influenced by environmental salinity. The comprehensive quantitative proteomic study performed here indicated that P. torquis responds to changing salinity and illumination conditions. Proteins in the electron-transfer chain were down-regulated at a suboptimal salinity level, TonB-dependent transporters increased in abundance under supra-optimal salinity and decreased under suboptimal salinity. In addition, several anaplerotic CO2 fixation proteins and three putative light sensing proteins that contain PAS and GAF domains became more abundant under illumination. Furthermore, central metabolic pathways (TCA and glycolysis) were also induced by both salinity stress and illumination. The data suggest that P. torquis responded to changes in both light energy and salinity to modulate membrane and central metabolic proteins that are involved in energy production as well as nutrient uptake and gliding motility processes that would be especially advantageous during the polar summer ice algal bloom.

Item Details

Item Type:Refereed Article
Keywords:Antarctic bacteria, proteomics, sea-ice, genomics, proteorhodopsin, survival strategies
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbial Ecology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:Feng, S (Mr Kevin Feng)
Author:Powell, SM (Dr Shane Powell)
Author:Wilson, R (Dr Richard Wilson)
Author:Bowman, JP (Associate Professor John Bowman)
ID Code:102149
Year Published:2015
Web of Science® Times Cited:6
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2015-07-31
Last Modified:2017-11-08
Downloads:0

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