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Direct coupling of a free-flow isotachophoresis (FFITP) device with electrospray ionization mass spectrometry (ESI-MS)

Citation

Park, JK and Campos, CDM and Neuzil, P and Abelmann, L and Guijt, RM and Manz, A, Direct coupling of a free-flow isotachophoresis (FFITP) device with electrospray ionization mass spectrometry (ESI-MS), Lab on A Chip: Miniaturisation for Chemistry and Biology, 15, (17) pp. 3495-502. ISSN 1473-0197 (2015) [Refereed Article]


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Copyright 2015 The Royal Society of Chemistry Licenced under Creative Commons Attribution 3.0 Unported (CC BY 3.0) http://creativecommons.org/licenses/by/3.0/

DOI: doi:10.1039/C5LC00523J

Abstract

We present the online coupling of a free-flow isotachophoresis (FFITP) device to an electrospray ionization mass spectrometer (ESI-MS) for continuous analysis without extensive sample preparation. Free-flow-electrophoresis techniques are used for continuous electrophoretic separations using an electric field applied perpendicular to the buffer and sample flow, with FFITP using a discontinuous electrolyte system to concurrently focus a target analyte and remove interferences. The online coupling of FFITP to ESI-MS decouples the separation and detection timeframe because the electrophoretic separation takes place perpendicular to the flow direction, which can be beneficial for monitoring (bio)chemical changes and/or extensive MSn studies. We demonstrated the coupling of FFITP with ESI-MS for simultaneous concentration of target analytes and sample clean-up. Furthermore, we show hydrodynamic control of the fluidic fraction injected into the MS, allowing for fluidically controlled scanning of the ITP window. Future applications of this approach are expected in monitoring biochemical changes and proteomics.


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Item Details

Item Type:Refereed Article
Keywords:free flow electrophoresis, mass spectrometry, isotachophoresis
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Guijt, RM (Dr Rosanne Guijt)
ID Code:101907
Year Published:2015
Web of Science® Times Cited:8
Deposited By:Pharmacy
Deposited On:2015-07-17
Last Modified:2017-10-30
Downloads:228 View Download Statistics

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