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Direct coupling of a free-flow isotachophoresis (FFITP) device with electrospray ionization mass spectrometry (ESI-MS)
Citation
Park, JK and Campos, CDM and Neuzil, P and Abelmann, L and Guijt, RM and Manz, A, Direct coupling of a free-flow isotachophoresis (FFITP) device with electrospray ionization mass spectrometry (ESI-MS), Lab on A Chip: Miniaturisation for Chemistry and Biology, 15, (17) pp. 3495-502. ISSN 1473-0197 (2015) [Refereed Article]
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Copyright Statement
Copyright 2015 The Royal Society of Chemistry Licenced under Creative Commons Attribution 3.0 Unported (CC BY 3.0) http://creativecommons.org/licenses/by/3.0/
Abstract
We present the online coupling of a free-flow isotachophoresis (FFITP) device to an electrospray ionization mass spectrometer (ESI-MS) for continuous analysis without extensive sample preparation. Free-flow-electrophoresis techniques are used for continuous electrophoretic separations using an electric field applied perpendicular to the buffer and sample flow, with FFITP using a discontinuous electrolyte system to concurrently focus a target analyte and remove interferences. The online coupling of FFITP to ESI-MS decouples the separation and detection timeframe because the electrophoretic separation takes place perpendicular to the flow direction, which can be beneficial for monitoring (bio)chemical changes and/or extensive MSn studies. We demonstrated the coupling of FFITP with ESI-MS for simultaneous concentration of target analytes and sample clean-up. Furthermore, we show hydrodynamic control of the fluidic fraction injected into the MS, allowing for fluidically controlled scanning of the ITP window. Future applications of this approach are expected in monitoring biochemical changes and proteomics.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | free flow electrophoresis, mass spectrometry, isotachophoresis |
| Research Division: | Chemical Sciences |
| Research Group: | Analytical Chemistry |
| Research Field: | Separation Science |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding Knowledge |
| Objective Field: | Expanding Knowledge in the Chemical Sciences |
| Author: | Guijt, RM (Dr Rosanne Guijt) |
| ID Code: | 101907 |
| Year Published: | 2015 |
| Web of Science® Times Cited: | 8 |
| Deposited By: | Pharmacy |
| Deposited On: | 2015-07-17 |
| Last Modified: | 2017-10-30 |
| Downloads: | 228 View Download Statistics |
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