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Distinct profiles of human embryonic stem cell metabolism and mitochondria identified by oxygen
Citation
Lees, JG and Rathjen, J and Sheedy, JR and Gardner, DK and Harvey, AJ, Distinct profiles of human embryonic stem cell metabolism and mitochondria identified by oxygen, Reproduction, 150, (4) pp. 367-382. ISSN 1470-1626 (2015) [Refereed Article]
Copyright Statement
Copyright 2015 Society for Reproduction and Fertility
Abstract
Oxygen is a powerful regulator of cell function and embryonic development. It has previously been determined that oxygen regulates human embryonic stem (hES) cell glycolytic and amino acid metabolism, but the effects on mitochondria are as yet unknown. Two hES cell lines (MEL1, MEL2) were analyzed to determine the role of 5% (physiological) and 20% (atmospheric) oxygen in regulating mitochondrial activity. In response to extended physiological oxygen culture, MEL2 hES cells displayed reduced mtDNA content, mitochondrial mass and expression of metabolic genes TFAM, NRF1, PPARa and MT-ND4. Furthermore, MEL2 hES cell glucose consumption, lactate production and amino acid turnover were elevated under physiological oxygen. In stark contrast, MEL1 hES cell amino acid and carbohydrate use and mitochondrial function were relatively unaltered in response to oxygen. Furthermore, differentiation kinetics were delayed in the MEL1 hES cell line following BMP4 treatment. Here we report the first incidence of metabolic dysfunction in a hES cell population, defined as a failure to respond to oxygen concentration through the modulation of metabolism, demonstrating that hES cells can be perturbed during culture despite exhibiting the defining characteristics of pluripotent cells. Collectively, these data reveal a central role for oxygen in the regulation of hES cell metabolism and mitochondrial function, whereby physiological oxygen promotes glucose flux and suppresses mitochondrial biogenesis and gene expression.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Research Division: | Biological Sciences |
| Research Group: | Biochemistry and cell biology |
| Research Field: | Cell development, proliferation and death |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding knowledge |
| Objective Field: | Expanding knowledge in the biological sciences |
| UTAS Author: | Rathjen, J (Dr Joy Rathjen) |
| ID Code: | 101901 |
| Year Published: | 2015 |
| Web of Science® Times Cited: | 21 |
| Deposited By: | Menzies Institute for Medical Research |
| Deposited On: | 2015-07-16 |
| Last Modified: | 2017-11-07 |
| Downloads: | 0 |
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