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Isolation via next-generation sequencing of microsatellites from the Tasmanian Macroalgae Lessonia corrugata (Lessoniaceae)

Citation

Durrant, HMS and Burridge, CP and Gardner, MG, Isolation via next-generation sequencing of microsatellites from the Tasmanian Macroalgae Lessonia corrugata (Lessoniaceae), Applications in Plant Sciences, 3, (7) Article 1500042. ISSN 2168-0450 (2015) [Contribution to Refereed Journal]


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Copyright Statement

2015 Durrant et al. Published by the Botanical Society of America. Licenced under Creative Commons Attribution-Non Commercial-Share alike 3.0 Australia (CC BY-NC-SA 3.0 AU)http://creativecommons.org/licenses/by-nc-sa/3.0/au/

DOI: doi:10.3732/apps.1500042

Abstract

Premise of the study: Microsatellite markers for the macroalgae Lessonia corrugata (Lessoniaceae) were developed, for the first time, to enable population genetic assessment of this important foundation species.

Methods and Results: Ion Torrent sequencing identified 16,622 loci, 29 of which were trialed in L. corrugata. Seven loci were found to be polymorphic and screened for variation in 76 individuals from two populations in Tasmania, Australia. Observed heterozygosity ranged from 0.086 to 0.686 (mean 0.386) and the number of alleles per locus ranged from two to five (mean 2.57). Heterozygosity was not signifi cantly different from expected values.

Conclusions: These loci can be used to study the population genetics of L. corrugata, a key habitat-forming species in the Tasmanian marine ecosystem, and will help to assess gene fl ow among spatially discrete populations such as those in marine protected areas.

Item Details

Item Type:Contribution to Refereed Journal
Keywords:ion torrent, Lessonia, Lessoniaceae, macroalgae, microsatellites, next-generation sequencing
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Population, Ecological and Evolutionary Genetics
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Durrant, HMS (Miss Halley Durrant)
Author:Burridge, CP (Dr Christopher Burridge)
ID Code:101894
Year Published:2015
Web of Science® Times Cited:1
Deposited By:Zoology
Deposited On:2015-07-16
Last Modified:2016-08-02
Downloads:514 View Download Statistics

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