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Isolation via next-generation sequencing of microsatellites from the Tasmanian Macroalgae Lessonia corrugata (Lessoniaceae)
Citation
Durrant, HMS and Burridge, CP and Gardner, MG, Isolation via next-generation sequencing of microsatellites from the Tasmanian Macroalgae Lessonia corrugata (Lessoniaceae), Applications in Plant Sciences, 3, (7) Article 1500042. ISSN 2168-0450 (2015) [Contribution to Refereed Journal]
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Copyright Statement
© 2015 Durrant et al. Published by the Botanical Society of America. Licenced under Creative Commons Attribution-Non Commercial-Share alike 3.0 Australia (CC BY-NC-SA 3.0 AU)http://creativecommons.org/licenses/by-nc-sa/3.0/au/
Abstract
• Premise of the study: Microsatellite markers for the macroalgae Lessonia corrugata (Lessoniaceae) were developed, for the
first time, to enable population genetic assessment of this important foundation species.
• Methods and Results: Ion Torrent sequencing identified 16,622 loci, 29 of which were trialed in L. corrugata. Seven loci were found to be polymorphic and screened for variation in 76 individuals from two populations in Tasmania, Australia. Observed heterozygosity ranged from 0.086 to 0.686 (mean 0.386) and the number of alleles per locus ranged from two to five (mean 2.57). Heterozygosity was not signifi cantly different from expected values.
• Conclusions: These loci can be used to study the population genetics of L. corrugata, a key habitat-forming species in the Tasmanian marine ecosystem, and will help to assess gene fl ow among spatially discrete populations such as those in marine protected areas.
Item Details
| Item Type: | Contribution to Refereed Journal |
|---|---|
| Keywords: | ion torrent, Lessonia, Lessoniaceae, macroalgae, microsatellites, next-generation sequencing |
| Research Division: | Biological Sciences |
| Research Group: | Genetics |
| Research Field: | Genetics not elsewhere classified |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding knowledge |
| Objective Field: | Expanding knowledge in the biological sciences |
| UTAS Author: | Durrant, HMS (Miss Halley Durrant) |
| UTAS Author: | Burridge, CP (Associate Professor Christopher Burridge) |
| ID Code: | 101894 |
| Year Published: | 2015 |
| Web of Science® Times Cited: | 2 |
| Deposited By: | Zoology |
| Deposited On: | 2015-07-16 |
| Last Modified: | 2016-08-02 |
| Downloads: | 597 View Download Statistics |
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