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Characterisation of three cDNA clones encoding different mRNAs for the precursor to the small subunit of wheat ribolosebisphosphate carboxylase
Citation
Smith, SM and Bedbrook, J and Speirs, J, Characterisation of three cDNA clones encoding different mRNAs for the precursor to the small subunit of wheat ribolosebisphosphate carboxylase, Nucleic Acids Research, 11, (24) pp. 8719-8734. ISSN 0305-1048 (1983) [Refereed Article]
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Copyright Statement
Copyright 1983 IRL Press Limited
DOI: doi:10.1093/nar/11.24.8719
Abstract
We have isolated and sequenced three cDNA clones for the nuclear encoded
precursor to the small subunit of the chloroplast enzyme, ribulose-
1,5-bisphosphate carboxylase of wheat. The nucleotide sequences of these
clones are different, indicating that they are probably derived from three
different mRNAs. This finding is consistent with the proposal that this
polypeptide is encoded by a multigene family in wheat, in support of
similar data reported by Broglie et al. (Bio/Technology 1:55-61, 1983).
We deduce that the mature small subunit polypeptide is comprised of 128
amino acids and that its precursor contains an N-terminal transit peptide
sequence. The sequences of both the mature small subunit and its transit
peptide differ at several positions from those determined by Broglie et al.
(1983) from a different wheat cultivar. Different wheat cultivars might
therefore contain different small subunit polypeptides. A comparison of
nucleotide and amino acid sequences of the small subunit from wheat, pea,
soybean 'and spinach shows that these sequences are not highly conserved,
particularly between monocotyledon and dicotyledon species.
Item Details
Item Type: | Refereed Article |
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Keywords: | cDNA, cloning, mRNA, ribulosebisphosphate carboxylase |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Smith, SM (Professor Steven Smith) |
ID Code: | 101644 |
Year Published: | 1983 |
Web of Science® Times Cited: | 29 |
Deposited By: | Plant Science |
Deposited On: | 2015-06-26 |
Last Modified: | 2015-09-24 |
Downloads: | 198 View Download Statistics |
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