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A nuclear-localized protein, KOLD SENSITIV-1, affects the expression of cold-responsive genes during prolonged chilling in Arabidopsis

Citation

Purdy, SJ and Bussell, JD and Nelson, DC and Villadsen, D and Smith, SM, A nuclear-localized protein, KOLD SENSITIV-1, affects the expression of cold-responsive genes during prolonged chilling in Arabidopsis, Journal of Plant Physiology, 168, (3) pp. 263-269. ISSN 0176-1617 (2011) [Refereed Article]

Copyright Statement

Copyright 2010 Elsevier GmbH.

DOI: doi:10.1016/j.jplph.2010.07.001

Abstract

Plants respond to cold by transcriptional and metabolic responses which underlie tolerance and acclimation mechanisms, but details at the molecular level are incomplete. Here we describe KOLD SENSITIV-1 (KOS1), a new gene required for responses to cold. KOS1 protein is predicted to have coiled-coil, Structural Maintenance of Chromosomes and nuclear-targeting domains. GFP-labeled KOS1 localizes to the nucleus. Null mutants could not be isolated but two independent knockdown T-DNA mutants were obtained. Growth and development of kos1 knockdown mutant plants was comparable to wild type when grown at 21 °C. However, when grown at 4 °C these mutants exhibited accelerated leaf yellowing and smaller rosette size than wild type. Quantitative RT-PCR revealed that in the cold kos1 mutants had reduced expression of cold-responsive transcripts COR15A, COR15B, BAM3 and AMY3. Metabolite profiling revealed that ascorbate levels were lower in the mutants in the cold relative to wild type. KOS1 therefore represents a new gene that influences the regulation of transcript and metabolite levels in response to prolonged chilling temperatures.

Item Details

Item Type:Refereed Article
Keywords:Arabidopsis thaliana, ascorbate, cold acclimation, gene expression, nuclear protein, starch
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Smith, SM (Professor Steven Smith)
ID Code:101635
Year Published:2011
Web of Science® Times Cited:8
Deposited By:Plant Science
Deposited On:2015-06-26
Last Modified:2018-10-05
Downloads:0

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