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A nuclear-localized protein, KOLD SENSITIV-1, affects the expression of cold-responsive genes during prolonged chilling in Arabidopsis
Citation
Purdy, SJ and Bussell, JD and Nelson, DC and Villadsen, D and Smith, SM, A nuclear-localized protein, KOLD SENSITIV-1, affects the expression of cold-responsive genes during prolonged chilling in Arabidopsis, Journal of Plant Physiology, 168, (3) pp. 263-269. ISSN 0176-1617 (2011) [Refereed Article]
Copyright Statement
Copyright 2010 Elsevier GmbH.
DOI: doi:10.1016/j.jplph.2010.07.001
Abstract
Plants respond to cold by transcriptional and metabolic responses which underlie tolerance and acclimation mechanisms, but details at the molecular level are incomplete. Here we describe KOLD SENSITIV-1 (KOS1), a new gene required for responses to cold. KOS1 protein is predicted to have coiled-coil, Structural Maintenance of Chromosomes and nuclear-targeting domains. GFP-labeled KOS1 localizes to the nucleus. Null mutants could not be isolated but two independent knockdown T-DNA mutants were obtained. Growth and development of kos1 knockdown mutant plants was comparable to wild type when grown at 21 °C. However, when grown at 4 °C these mutants exhibited accelerated leaf yellowing and smaller rosette size than wild type. Quantitative RT-PCR revealed that in the cold kos1 mutants had reduced expression of cold-responsive transcripts COR15A, COR15B, BAM3 and AMY3. Metabolite profiling revealed that ascorbate levels were lower in the mutants in the cold relative to wild type. KOS1 therefore represents a new gene that influences the regulation of transcript and metabolite levels in response to prolonged chilling temperatures.
Item Details
Item Type: | Refereed Article |
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Keywords: | Arabidopsis thaliana, ascorbate, cold acclimation, gene expression, nuclear protein, starch |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Smith, SM (Professor Steven Smith) |
ID Code: | 101635 |
Year Published: | 2011 |
Web of Science® Times Cited: | 8 |
Deposited By: | Plant Science |
Deposited On: | 2015-06-26 |
Last Modified: | 2018-10-05 |
Downloads: | 0 |
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