eCite Digital Repository

A nuclear-localized protein, KOLD SENSITIV-1, affects the expression of cold-responsive genes during prolonged chilling in Arabidopsis

Citation

Purdy, SJ and Bussell, JD and Nelson, DC and Villadsen, D and Smith, SM, A nuclear-localized protein, KOLD SENSITIV-1, affects the expression of cold-responsive genes during prolonged chilling in Arabidopsis, Journal of Plant Physiology, 168, (3) pp. 263-269. ISSN 0176-1617 (2011) [Refereed Article]

Copyright Statement

Copyright 2010 Elsevier GmbH.

DOI: doi:10.1016/j.jplph.2010.07.001

Abstract

Plants respond to cold by transcriptional and metabolic responses which underlie tolerance and acclimation mechanisms, but details at the molecular level are incomplete. Here we describe KOLD SENSITIV-1 (KOS1), a new gene required for responses to cold. KOS1 protein is predicted to have coiled-coil, Structural Maintenance of Chromosomes and nuclear-targeting domains. GFP-labeled KOS1 localizes to the nucleus. Null mutants could not be isolated but two independent knockdown T-DNA mutants were obtained. Growth and development of kos1 knockdown mutant plants was comparable to wild type when grown at 21C. However, when grown at 4C these mutants exhibited accelerated leaf yellowing and smaller rosette size than wild type. Quantitative RT-PCR revealed that in the cold kos1 mutants had reduced expression of cold-responsive transcripts COR15A, COR15B, BAM3 and AMY3. Metabolite profiling revealed that ascorbate levels were lower in the mutants in the cold relative to wild type. KOS1 therefore represents a new gene that influences the regulation of transcript and metabolite levels in response to prolonged chilling temperatures.

Item Details

Item Type:Refereed Article
Keywords:Arabidopsis thaliana, ascorbate, cold acclimation, gene expression, nuclear protein, starch
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Smith, SM (Professor Steven Smith)
ID Code:101635
Year Published:2011
Web of Science® Times Cited:4
Deposited By:Plant Science
Deposited On:2015-06-26
Last Modified:2018-07-11
Downloads:0

Repository Staff Only: item control page