eCite Digital Repository
Synthesis of the small subunit of ribulose-bisphosphate carboxylase from genes cloned into plasmids containing the SP6 promoter
Citation
Anderson, S and Smith, SM, Synthesis of the small subunit of ribulose-bisphosphate carboxylase from genes cloned into plasmids containing the SP6 promoter, Biochemical Journal, 240, (3) pp. 709-715. ISSN 0264-6021 (1986) [Refereed Article]
Copyright Statement
Copyright 1986 London: The Biochemical Society
Abstract
DNA sequences encoding ribulose 1,5-bisphosphate carboxylase small subunit precursor from Pisum
sativum L. have been transcribed from plasmids containing the SP6 promoter, and translated in a wheat
germ cell-free system. The small subunit precursor polypeptide, its N-terminal leader sequence (transit
peptide) and the mature small subunit have each been synthesized independently from three different
plasmid constructs. The precursor polypeptide is imported into isolated pea chloroplasts and processed to
the mature small subunit by a stromal proteinase. The mature polypeptide is neither imported, nor subject
to proteolysis by stromal extracts. The transit peptide alone is very rapidly degraded by a stromal proteinase
activity which can be inhibited by EDTA or 1,10-phenanthroline. The use of these gene constructs helps
to establish the crucial role of the transit peptide in protein import into the chloroplast.
Item Details
Item Type: | Refereed Article |
---|---|
Keywords: | ribulose 1,5-bisphosphate carboxylase, Pisum sativum L., SP6, cloning |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Smith, SM (Professor Steven Smith) |
ID Code: | 101609 |
Year Published: | 1986 |
Web of Science® Times Cited: | 74 |
Deposited By: | Plant Science |
Deposited On: | 2015-06-26 |
Last Modified: | 2016-02-22 |
Downloads: | 0 |
Repository Staff Only: item control page