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Glyoxysomal malate synthase of cucumber: molecular cloning of a cDNA and regulation of enzyme synthesis during germination
Citation
Smith, SM and Leaver, CJ, Glyoxysomal malate synthase of cucumber: molecular cloning of a cDNA and regulation of enzyme synthesis during germination, Plant Physiology, 81, (3) pp. 762-767. ISSN 0032-0889 (1986) [Refereed Article]
Copyright Statement
Copyright 1986 American Society of Plant Biologists.
Abstract
A cDNA clone for the glyoxysomal enzyme malate synthase was
isolated from a cDNA library made with polyadenylated RNA from the
cotyledons of germinating Cucumis sativus L. This cloned DNA sequence
was used as a probe to characterize changes in the amounts of malate
synthase gene transcripts in cotyledons of cucumber seeds grown both in
the light and in the dark. Malate synthase gene transcripts increase in
amount to a peak at day 3 or day 4, and thereafter decline. In the light,
this rate of decline is significantly greater than in the dark. Measurement
of the changes in the amounts of malate synthase by assaying enzyme
activity directly, and by immunological reaction with a specific antiserum
indicate that the developmentally regulated synthesis of malate synthase
in germinating cucumber is brought about primarily by changes in the
amount of malate synthase gene transcripts, rather than through a control
of translation. Similarly, the effect of light on the amount of malate
synthase correlates precisely with its effect on the abundance of malate
synthase gene transcripts.
Item Details
Item Type: | Refereed Article |
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Keywords: | glyoxysomal malate synthase, molecular cloning, cDNA, germination |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Smith, SM (Professor Steven Smith) |
ID Code: | 101608 |
Year Published: | 1986 |
Web of Science® Times Cited: | 53 |
Deposited By: | Plant Science |
Deposited On: | 2015-06-26 |
Last Modified: | 2015-09-24 |
Downloads: | 0 |
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