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The malate synthase gene of cucumber
journal contribution
posted on 2023-05-18, 11:31 authored by Graham, IA, Smith, LM, Brown, JWS, Leaver, CJ, Steven SmithSteven SmithThe complete sequences of a full-length cDNA clone and a genomic clone encoding the Cucumis sativus glyoxysomal enzyme malate synthase, have been determined. The sequences have enabled us to identify putative control regions at the 5' end of the gene, three introns, and possible alternative polyadenylation sites at the 3' end. The deduced amino acid sequence predicts a polypeptide of 64 961 molecular weight, which has 48 ~o identity with that of Escherichia coli. Comparison of the sequence of malate synthase from cucumber with that from E. coli and with other glyoxysomal and peroxisomal enzymes, shows that a conserved C-terminal tripeptide is a common feature of those enzymes imported into microbodies.
History
Publication title
Plant Molecular BiologyVolume
13Issue
6Pagination
673-684ISSN
0167-4412Department/School
School of Natural SciencesPublisher
Kluwer Academic PublPlace of publication
Van Godewijckstraat 30, Dordrecht, Netherlands, 3311 GzRights statement
Copyright 1989 Kluwer Academic PublishersRepository Status
- Restricted