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Methylation of ribosomal RNA genes in Petunia hybrida plants, callus cultures and regenerated shoots
Citation
Anderson, S and Lewis-Smth, AC and Smith, SM, Methylation of ribosomal RNA genes in Petunia hybrida plants, callus cultures and regenerated shoots, Plant Cell Reports, 8, (9) pp. 554-557. ISSN 0721-7714 (1990) [Refereed Article]
Copyright Statement
Copyright 1990 Springer-Verlag
Abstract
The extent of methylation of the 18S-25S ribosomal
RNA genes of Petunia hybrida has been investigated
using methylation-sensitive restriction endonucleases
and Southern hybridisation. The genes in leaves,
ovaries, petals, stigmas, anthers, and seed are
methylated to a similar extent, but those in roots
are hypomethylated. Genes in adventitious roots
formed from stem cuttings are also hypomethylated.
Genes in callus cultures initiated from leaf discs
vary widely in the extent of methylation, but the
pattern obtained is stable during culture. Callus
cells containing hypomethylated genes can give rise
to shoots in which the extent of methylation has
reverted to that of the original leaf explant. These
results demonstrate the striking plasticity of
ribosomal RNA gene methylation in Petunia.
Item Details
Item Type: | Refereed Article |
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Keywords: | methylation, ribosomal RNA, Petunia hybrida, callus cultures |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Smith, SM (Professor Steven Smith) |
ID Code: | 101603 |
Year Published: | 1990 |
Web of Science® Times Cited: | 21 |
Deposited By: | Plant Science |
Deposited On: | 2015-06-26 |
Last Modified: | 2015-08-25 |
Downloads: | 0 |
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