University of Tasmania
Browse

File(s) under permanent embargo

Methylation of ribosomal RNA genes in Petunia hybrida plants, callus cultures and regenerated shoots

journal contribution
posted on 2023-05-18, 11:31 authored by Anderson, S, Lewis-Smth, AC, Steven SmithSteven Smith
The extent of methylation of the 18S-25S ribosomal RNA genes of Petunia hybrida has been investigated using methylation-sensitive restriction endonucleases and Southern hybridisation. The genes in leaves, ovaries, petals, stigmas, anthers, and seed are methylated to a similar extent, but those in roots are hypomethylated. Genes in adventitious roots formed from stem cuttings are also hypomethylated. Genes in callus cultures initiated from leaf discs vary widely in the extent of methylation, but the pattern obtained is stable during culture. Callus cells containing hypomethylated genes can give rise to shoots in which the extent of methylation has reverted to that of the original leaf explant. These results demonstrate the striking plasticity of ribosomal RNA gene methylation in Petunia.

History

Publication title

Plant Cell Reports

Volume

8

Issue

9

Pagination

554-557

ISSN

0721-7714

Department/School

School of Natural Sciences

Publisher

Springer-Verlag

Place of publication

175 Fifth Ave, New York, USA, Ny, 10010

Rights statement

Copyright 1990 Springer-Verlag

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the biological sciences

Usage metrics

    University Of Tasmania

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC