eCite Digital Repository
Genetic and chromosomal variation in Petunia hybrida plants regenerated from protoplast and callus cultures
Citation
Lewis-Smith, AC and Chamberlain, M and Smith, SM, Genetic and chromosomal variation in Petunia hybrida plants regenerated from protoplast and callus cultures, Biologia Plantarum, 32, (4) pp. 247-255. ISSN 0006-3134 (1990) [Refereed Article]
Copyright Statement
Copyright unknown
Abstract
Plants regenerated from callus cultures derived from leaf discs and mesophyll protoplasts ofPetunia hybrida cv. Rose of Heaven exhibit a high frequency of genetic and chromosomal variation. Of twelve leaf disc-derived plants examined, only three had the normal diploid chromosome number (2n=14) while seven were tetraploid and two were aneuploid (16 and 27 chromosomes). Of seventeen plants derived from two protoplasts, none had the diploid chromosome number. Most had 28 chromosomes, one 29, two 27, one 26 and one had variable numbers (14–28) in different root tip cells. In all cases aneuploidy was associated with developmental abnormality. In addition, heritable differences in growth, morphology and flower pigmentation were observed in callus-derived tetraploids and diploids, including one diploid which differed from parent plants in at least four characters. These results are discussed in terms of the importance ofPetunia in genetics research and for studies of somaclonal variation.
Item Details
Item Type: | Refereed Article |
---|---|
Keywords: | Petunia hybrida, regeneration, chromosomal variation |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Smith, SM (Professor Steven Smith) |
ID Code: | 101600 |
Year Published: | 1990 |
Web of Science® Times Cited: | 6 |
Deposited By: | Plant Science |
Deposited On: | 2015-06-26 |
Last Modified: | 2018-10-05 |
Downloads: | 0 |
Repository Staff Only: item control page