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Recombinant aequorin as a probe for cytosolic free Ca 2+ in Escherichia coli

Citation

Knight, MR and Campbell, AK and Smith, SM and Trewavas, AJ, Recombinant aequorin as a probe for cytosolic free Ca 2+ in Escherichia coli, FEBS Letters, 282, (2) pp. 405-408. ISSN 0014-5793 (1991) [Refereed Article]

Copyright Statement

Copyright 1991 Federation of European Biochemical Societies

DOI: doi:10.1016/0014-5793(91)80524-7

Abstract

We describe a novel and simple method for the measurement of bacterial cytosolic free calcium ([Ca2+]i) using recombinant acquorin reconstituted within live bacterial cells. Using this method we have measured the effects of external calcium, complement, phagocytosis and antibiotics on the [Ca2+]i of Escherichia coli. In principle this method should be applicable to any genetically transformable organism and should suffer fewer problems than fluorescent dyes for subcellular calcium measurement. © 1991.

Item Details

Item Type:Refereed Article
Keywords:aequorin, reconsitution, coelenteraxine, E. coli complement, phaocytosis, antibiotic, cytoplasmic Ca2+
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Smith, SM (Professor Steven Smith)
ID Code:101594
Year Published:1991
Web of Science® Times Cited:68
Deposited By:Plant Science
Deposited On:2015-06-26
Last Modified:2015-09-24
Downloads:0

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