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Recombinant aequorin as a probe for cytosolic free Ca 2+ in Escherichia coli
Citation
Knight, MR and Campbell, AK and Smith, SM and Trewavas, AJ, Recombinant aequorin as a probe for cytosolic free Ca 2+ in Escherichia coli, FEBS Letters, 282, (2) pp. 405-408. ISSN 0014-5793 (1991) [Refereed Article]
Copyright Statement
Copyright 1991 Federation of European Biochemical Societies
DOI: doi:10.1016/0014-5793(91)80524-7
Abstract
We describe a novel and simple method for the measurement of bacterial cytosolic free calcium ([Ca2+]i) using recombinant acquorin reconstituted within live bacterial cells. Using this method we have measured the effects of external calcium, complement, phagocytosis and antibiotics on the [Ca2+]i of Escherichia coli. In principle this method should be applicable to any genetically transformable organism and should suffer fewer problems than fluorescent dyes for subcellular calcium measurement. © 1991.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | aequorin, reconsitution, coelenteraxine, E. coli complement, phaocytosis, antibiotic, cytoplasmic Ca2+ |
| Research Division: | Biological Sciences |
| Research Group: | Plant Biology |
| Research Field: | Plant Physiology |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding Knowledge |
| Objective Field: | Expanding Knowledge in the Biological Sciences |
| Author: | Smith, SM (Professor Steven Smith) |
| ID Code: | 101594 |
| Year Published: | 1991 |
| Web of Science® Times Cited: | 68 |
| Deposited By: | Plant Science |
| Deposited On: | 2015-06-26 |
| Last Modified: | 2015-09-24 |
| Downloads: | 0 |
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