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lnduction of malate synthase gene expression in senescent and detached organs of cucumber
Citation
Graham, IA and Leaver, CJ and Smith, SM, lnduction of malate synthase gene expression in senescent and detached organs of cucumber, Plant Cell, 4 pp. 349-357. ISSN 1040-4651 (1992) [Refereed Article]
Copyright Statement
Copyright 1993 by American Society of Plant Biologists
Official URL: http://www.plantcell.org/content/4/3/349.abstract
Abstract
Expression of the malate synthase (MS) gene is activated in cotyledons of cucumber seedlings during postgerminative
growth and then repressed as the cotyledons become photosynthetic. MS gene expression is subsequently reactivated
in the cotyledons as they senesce a few weeks later. In situ hybridization revealed that MS RNA is distributed throughout
the organ during postgerminative growth and senescence, showing that the same cells express the gene at different
stages of development. MS RNA also appears in senescing leaves and petals of cucumber plants. In addition, we found
that MS RNA appears in mature expanded leaves and roots when they are removed from the plant and incubated in darkness
for several days, thus providing a potential experimental system for the manipulation of MS gene expression. Leaves
from transgenic Nicotiana plumbaginifolia containing the cucumber MS promoter fused to the beta-glucuronidase (GUS)
reporter gene accumulated GUS activity when detached, demonstrating an activation of transcription from the MS promoter
following leaf excision. These results are discussed in terms of the metabolic regulation of MS gene expression.
Item Details
Item Type: | Refereed Article |
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Keywords: | malate synthase, cucumber, senescence |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Smith, SM (Professor Steven Smith) |
ID Code: | 101593 |
Year Published: | 1992 |
Deposited By: | Plant Science |
Deposited On: | 2015-06-26 |
Last Modified: | 2015-09-24 |
Downloads: | 0 |
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