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lnduction of malate synthase gene expression in senescent and detached organs of cucumber

journal contribution
posted on 2023-05-18, 11:30 authored by Graham, IA, Leaver, CJ, Steven SmithSteven Smith
Expression of the malate synthase (MS) gene is activated in cotyledons of cucumber seedlings during postgerminative growth and then repressed as the cotyledons become photosynthetic. MS gene expression is subsequently reactivated in the cotyledons as they senesce a few weeks later. In situ hybridization revealed that MS RNA is distributed throughout the organ during postgerminative growth and senescence, showing that the same cells express the gene at different stages of development. MS RNA also appears in senescing leaves and petals of cucumber plants. In addition, we found that MS RNA appears in mature expanded leaves and roots when they are removed from the plant and incubated in darkness for several days, thus providing a potential experimental system for the manipulation of MS gene expression. Leaves from transgenic Nicotiana plumbaginifolia containing the cucumber MS promoter fused to the beta-glucuronidase (GUS) reporter gene accumulated GUS activity when detached, demonstrating an activation of transcription from the MS promoter following leaf excision. These results are discussed in terms of the metabolic regulation of MS gene expression.

History

Publication title

Plant Cell

Volume

4

Pagination

349-357

ISSN

1040-4651

Department/School

School of Natural Sciences

Publisher

Amer Soc Plant Biologists

Place of publication

15501 Monona Drive, Rockville, USA, Md, 20855

Rights statement

Copyright 1993 by American Society of Plant Biologists

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the biological sciences

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