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Disproportionating enzyme (4-a-Glucanotransferase; EC 2.4.1.25) of potato
Citation
Takaha, T and Yanase, M and Okada, S and Smith, SM, Disproportionating enzyme (4-a-Glucanotransferase; EC 2.4.1.25) of potato, Journal of Biological Chemistry, 268, (2) pp. 1391-1396. ISSN 0021-9258 (1993) [Refereed Article]
Copyright Statement
Copyright 1993 The American Society for Biochemistry and Molecular Biology, Inc
Official URL: http://www.jbc.org/content/268/2/1391.abstract
Abstract
Disproportionating enzyme (D-enzyme, 4-a-glucanotransferase;
EC 2.4.1.25) has been purified to homogeneity
from potato tubers and its activity characterized.
The enzyme catalyzes the transfer of maltooligosaccharides
from one 1,4-a-~-glucan molecule to
another, or to glucose. Maltooligosaccharides are effective
donor molecules, but short chain amylose and
amylopectin may also function as donors. Enzyme activity
is not affected by inorganic phosphate, S-phosphoglycerate,
or hexose phosphates. A cDNA clone
encoding the enzyme was isolated using oligonucleotide
probes derived from partial peptide sequences of the
purified enzyme. The identity of the cDNA clone was
confirmed by expression in Escherichia coli resulting
in D-enzyme activity. The amino acid sequence deduced
from the cDNA showing significant homology with
a 4-a-glucanotransferase from Streptococcus. The deduced
sequence indicates the presence of an aminoterminal
plastid transit peptide pf 52 amino acid residues
and a mature polypeptide of 524 residues. D-enzyme
mRNA is present in leaves, stems, roots, and
stolons but is most abundant in developing and mature
tubers. The amount of mRNA in leaves increases in
response to light and to- sucrose added to the medium.
These results are discussed in terms of the function of
D-enzyme in potato starch metabolism.
Item Details
Item Type: | Refereed Article |
---|---|
Keywords: | d-enzyme, 4-alpha-glucanotransferase, potato |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Smith, SM (Professor Steven Smith) |
ID Code: | 101591 |
Year Published: | 1993 |
Web of Science® Times Cited: | 97 |
Deposited By: | Plant Science |
Deposited On: | 2015-06-26 |
Last Modified: | 2015-09-21 |
Downloads: | 0 |
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