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Expression of a single gene encoding microbody NAD-malate dehydrogenase during glyoxysome and peroxisome development in cucumber
Citation
Kim, D-J and Smith, SM, Expression of a single gene encoding microbody NAD-malate dehydrogenase during glyoxysome and peroxisome development in cucumber, Plant Molecular Biology, 26, (6) pp. 1833-1841. ISSN 0167-4412 (1994) [Refereed Article]
Copyright Statement
Copyright 1994 Kluwer Academic Publishers
Official URL: http://link.springer.com/article/10.1007%2FBF00019...
Abstract
A full-length cDNA clone encoding microbody NAD +-dependent malate dehydrogenase (MDH) of
cucumber has been isolated. The deduced amino acid sequence is 97 ~o identical to glyoxysomal MDH
(gMDH) of watermelon, including the amino terminal putative transit peptide. The cucumber genome
contains only a single copy of this gene. Expression of this mdh gene increases dramatically in cotyledons
during the few days immediately following seed imbibition, in parallel with genes encoding isocitrate
lyase (ICL) and malate synthase (MS), two glyoxylate cycle enzymes. The level of MDH, ICL and MS
mRNAs then declines, but then MDH mRNA increases again together with that of peroxisomal NAD + -
dependent hydroxypyruvate reductase (HPR). The mdh gene is also expressed during cotyledon senescence,
together with hpr, icl and ms genes. These results indicate that a single gene encodes MDH which
functions in both glyoxysomes and peroxisomes. In contrast to icI and ms genes, expression of the mdh
gene is not activated by incubating detached green cotyledons in the dark, nor is it affected by exogenous
sucrose in the incubation medium. The function of this microbody MDH and the regulation of its
synthesis are discussed.
Item Details
Item Type: | Refereed Article |
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Keywords: | cucumis sativus, germination, glyoxysome, NAD-malate dehydrogenase, peroxisome, senescence |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Smith, SM (Professor Steven Smith) |
ID Code: | 101588 |
Year Published: | 1994 |
Web of Science® Times Cited: | 41 |
Deposited By: | Plant Science |
Deposited On: | 2015-06-26 |
Last Modified: | 2015-09-21 |
Downloads: | 0 |
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