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Distinct cis-acting elements direct the germination and sugar responses of the cucumber malate synthase gene

Citation

Sarah, CJ and Graham, IA and Reynolds, SJ and Leaver, CJ and Smith, SM, Distinct cis-acting elements direct the germination and sugar responses of the cucumber malate synthase gene, Molecular and General Genetics, 250, (2) pp. 153-161. ISSN 0026-8925 (1996) [Refereed Article]

Copyright Statement

Copyright 1996 Springer-Verlag

DOI: doi:10.1007/BF02174174

Abstract

The malate synthase gene (ms) promoter in cucumber (Cucumis sativus L.) was investigated with the aim of distinguishing DNA sequences mediating regulation of gene expression by sugar, and expression following seed germination. Promoter deletions were constructed and their ability to direct expression of theβ-glucuronidase (gus) reporter gene was investigated in transgenicNicotiana plumbaginifolia. Gene expression was assayed in germinating seeds and developing seedlings (the germination response) and in seedlings transferred from light into darkness with and without sucrose (the sugar response). As progressively more of the promoter was deleted from the 5′ end, first the sugar response and then the germination response was lost. Thus, distinct regions of the promoter are required for carbohydrate control and for regulation of gene expression in response to germination. Sequence comparisons of thems promoter with that of the isocitrate lyase gene (icl) of cucumber have previously identified four IMH (ICL-MS Homology) sequences. One such sequence, IMH2, is shown here to be implicated in the sugar response of thems gene. The 17 bp sequence, which when deleted from thems gene results in loss of the germination response, contains a 14 bp sequence which is similar to a sequence in theicl promoter, which we refer to as IMH5. Furthermore, this sequence has similarity withamdI9-like sequences in filamentous fungi, which conferfacB-mediated acetate inducibility on several genes, including those encoding ICL and MS.

Item Details

Item Type:Refereed Article
Keywords:cucumber (Cucumis sativus L.), malate synthase, glyoxylate cycle, gene transcription, metabolic regulation
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Smith, SM (Professor Steven Smith)
ID Code:101584
Year Published:1996
Web of Science® Times Cited:31
Deposited By:Plant Science
Deposited On:2015-06-26
Last Modified:2015-08-25
Downloads:0

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