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Potato D-enzyme catalyzes the cyclization of amylose to produce cycloamylose, a novel cyclic glucan

Citation

Takaha, T and Yanase, M and Takata, H and Okada, S and Smith, SM, Potato D-enzyme catalyzes the cyclization of amylose to produce cycloamylose, a novel cyclic glucan, Journal of Biological Chemistry, 271, (6) pp. 2902-2908. ISSN 0021-9258 (1996) [Refereed Article]

Copyright Statement

Copyright 1996 The American Society for Biochemistry and Molecular Biology Inc

DOI: doi:10.1074/jbc.271.6.2902

Abstract

Potato D-enzyme was purified from recombinant Escherichia coli, and its action on synthetic amylose (average Mr of 320,000) was analyzed. D-enzyme treatment resulted in a decrease in the ability of the amylose to form a blue complex with iodine. Analysis of the products indicated that the enzyme catalyzes an intramolecular transglycosylation reaction on amylose to produce cyclic α-1,4-glucan (cycloamylose). Confirmation of the cyclic structure was achieved by demonstrating the absence of reducing and nonreducing ends, resistance to hydrolysis by glucoamylase (an exoamylase), and by "time of flight" mass spectrometry. The degree of polymerization of cycloamylose products was determined by time of flight mass spectrometry analysis and by high-performance anion-exchange chromatography following partial acid hydrolysis of purified cycloamylose molecules and was found to range from 17 to several hundred. The yield of cycloamylose increased with time and reached >95%. D-enzyme did not act upon purified cycloamylose, but if glucose was added as an acceptor molecule, smaller cyclic and linear molecules were produced. The mechanism of the cyclization reaction, the possible role of the enzyme in starch metabolism, and the potential applications for cycloamylose are discussed.

Item Details

Item Type:Refereed Article
Keywords:d-enzyme, amylose, cucloamylose, cyclic glucan
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Smith, SM (Professor Steven Smith)
ID Code:101583
Year Published:1996
Web of Science® Times Cited:140
Deposited By:Plant Science
Deposited On:2015-06-26
Last Modified:2015-09-21
Downloads:0

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