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Potato D-enzyme catalyzes the cyclization of amylose to produce cycloamylose, a novel cyclic glucan
Citation
Takaha, T and Yanase, M and Takata, H and Okada, S and Smith, SM, Potato D-enzyme catalyzes the cyclization of amylose to produce cycloamylose, a novel cyclic glucan, Journal of Biological Chemistry, 271, (6) pp. 2902-2908. ISSN 0021-9258 (1996) [Refereed Article]
Copyright Statement
Copyright 1996 The American Society for Biochemistry and Molecular Biology Inc
DOI: doi:10.1074/jbc.271.6.2902
Abstract
Potato D-enzyme was purified from recombinant Escherichia coli, and its action on synthetic amylose (average Mr of 320,000) was analyzed. D-enzyme treatment resulted in a decrease in the ability of the amylose to form a blue complex with iodine. Analysis of the products indicated that the enzyme catalyzes an intramolecular transglycosylation reaction on amylose to produce cyclic α-1,4-glucan (cycloamylose). Confirmation of the cyclic structure was achieved by demonstrating the absence of reducing and nonreducing ends, resistance to hydrolysis by glucoamylase (an exoamylase), and by "time of flight" mass spectrometry. The degree of polymerization of cycloamylose products was determined by time of flight mass spectrometry analysis and by high-performance anion-exchange chromatography following partial acid hydrolysis of purified cycloamylose molecules and was found to range from 17 to several hundred. The yield of cycloamylose increased with time and reached >95%. D-enzyme did not act upon purified cycloamylose, but if glucose was added as an acceptor molecule, smaller cyclic and linear molecules were produced. The mechanism of the cyclization reaction, the possible role of the enzyme in starch metabolism, and the potential applications for cycloamylose are discussed.
Item Details
Item Type: | Refereed Article |
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Keywords: | d-enzyme, amylose, cucloamylose, cyclic glucan |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Smith, SM (Professor Steven Smith) |
ID Code: | 101583 |
Year Published: | 1996 |
Web of Science® Times Cited: | 162 |
Deposited By: | Plant Science |
Deposited On: | 2015-06-26 |
Last Modified: | 2015-09-21 |
Downloads: | 0 |
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