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Glycoxylate cycle enzyme activities are induced in senescent pumpkin fruits
Citation
Pistelli, L and Nieri, B and Smith, SM and Alpi, A and de Bellis, L, Glycoxylate cycle enzyme activities are induced in senescent pumpkin fruits, Plant Science, 119, (1-2) pp. 23-29. ISSN 0168-9452 (1996) [Refereed Article]
Copyright Statement
Copyright 1996 Elsevier Science Ireland Ltd.
DOI: doi:10.1016/0168-9452(96)04455-X
Abstract
The presence of isocitrate lyase (EC 4.1.3.1) and malate synthase (EC 4.1.3.2) was investigated in detached pumpkin fruits (Cucurbita pepo L., var. Alberello di Sarzana). After incubation of pumpkin fruit slices in permanent darkness, the enzyme activities appeared after only 1 day and then declined to zero at day 6. Catalase (EC 1.11.1.6) specific activity increased during the first 2 days of incubation and decreased thereafter. Hydroxypyruvate reductase (EC 1.1.1.81) and 3-hydroxyacyl CoA dehydrogenase (E.C. 1.1.1.35) specific activities changed very little, but NADP+-dependent isocitrate dehydrogenase (E.C. 1.1.1.42) and fumarase (E.C. 4.2.1.2) specific activities increased during the first day and declined thereafter. Sucrose density gradient fractionation of cell organelles showed that isocitrate lyase and malate synthase are localized in peroxisomal fractions. The presence of the two key enzymes of the glyoxylate cycle was confirmed by immunoblotting, both in crude extracts and in peroxisomal fractions. It is concluded that following detachment and dark incubation, a transition from peroxisomes to glyoxysomes occurs in pumpkin fruit.
Item Details
Item Type: | Refereed Article |
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Keywords: | fruits, glyoxylate cycle, peroxisomes, pumpkin, senescence |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Smith, SM (Professor Steven Smith) |
ID Code: | 101581 |
Year Published: | 1996 |
Web of Science® Times Cited: | 19 |
Deposited By: | Plant Science |
Deposited On: | 2015-06-26 |
Last Modified: | 2015-09-21 |
Downloads: | 0 |
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