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Normal starch content and composition in tubers of antisense potato plants lacking D-enzyme (4-α-glucanotransferase)
Citation
Takaha, T and Critchley, J and Okada, S and Smith, SM, Normal starch content and composition in tubers of antisense potato plants lacking D-enzyme (4-α-glucanotransferase), Planta, 205, (3) pp. 445-451. ISSN 0032-0935 (1998) [Refereed Article]
Copyright Statement
Copyright 1998 Springer-Verlag
DOI: doi:10.1007/s004250050342
Abstract
Transgenic potato (Solanum tuberosum L.) plants were created with sense and antisense copies of the potato D-enzyme (disproportionating enzyme; EC 2.4.1.25) cDNA linked to patatin and cauliflower mosaic virus 35 S promoters, and screened for D-enzyme activity in tubers. Transformants with sense constructs mostly had wild type D-enzyme activity but two plants had only about 1% wild-type activity. Transformants with antisense constructs had activity ranging from 90% to about 1% of wild type. Three 35 S antisense plants with very low activity were analysed in detail. Western blot analysis showed that D-enzyme was present in greatly reduced amounts in tubers and in leaves, whereas plastidic starch phosphorylase (EC 2.4.1.1) was unaffected. The lack of D-enzyme resulted in slow plant growth but development was otherwise apparently normal. Furthermore, the starch content of tubers was not appreciably altered in amount, proportion of amylose, molecular weight of debranched amylopectin, or branch chain length, despite the lack of D-enzyme. These results do not indicate a direct requirement for D-enzyme in the synthesis and accumulation of storage starch in tubers. The results are discussed in terms of the known reactions catalysed by D-enzyme and possible involvement of D-enzyme in starch metabolism.
Item Details
Item Type: | Refereed Article |
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Keywords: | antisense inhibition, D-enzyme, glucanotransferase, Solanum tuberosum, starch metabolism |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Smith, SM (Professor Steven Smith) |
ID Code: | 101572 |
Year Published: | 1998 |
Web of Science® Times Cited: | 36 |
Deposited By: | Plant Science |
Deposited On: | 2015-06-26 |
Last Modified: | 2015-08-25 |
Downloads: | 0 |
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