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Cyclic glucans produced by the intramolecular transglycosylation activity of potato D-enzyme on amylopectin

Citation

Takaha, T and Yanase, M and Takata, H and Okada, S and Smith, SM, Cyclic glucans produced by the intramolecular transglycosylation activity of potato D-enzyme on amylopectin, Biochemical and Biophysical Research Communications, 247, (2) pp. 493-497. ISSN 0006-291X (1998) [Refereed Article]

Copyright Statement

Copyright 1998 Academic Press

DOI: doi:10.1006/bbrc.1998.8817

Abstract

Potato D-enzyme catalyses an intramolecular transglycosylation reaction on amylose to produce cycloamylose, a novel cyclic α-1,4 glucan. To determine if a similar activity could be observed with a high molecular weight branched substrate, recombinant potato D-enzyme was incubated with amylopectin. The substrate was converted into two fractions of lower molecular mass. Fraction I comprised 15% cyclic molecules of which the majority contained both α-1,4 and α-1,6 links. These were shown to be branched molecules with branches shorter than those in amylopectin. Fraction II comprised 80% cyclic molecules of which the majority contained only α-1,4 links (cycloamylose). Since fraction II appeared before fraction I, we propose that D-enzyme first catalysed the cyclisation of the outer side chains of amylopectin and then the cyclisation of inner chains to produce branched clusters. These results demonstrate that D-enzyme can catalyse the transfer of branched glucans, and suggest novel ways in which it may participate in starch metabolism in plants.

Item Details

Item Type:Refereed Article
Keywords:cyclic glucans, intramolecular transglycosylation, potato d-enzyme, amylopectin, 4-a-glucanotransferase
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Smith, SM (Professor Steven Smith)
ID Code:101570
Year Published:1998
Web of Science® Times Cited:35
Deposited By:Plant Science
Deposited On:2015-06-26
Last Modified:2015-09-21
Downloads:0

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