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Cyclic glucans produced by the intramolecular transglycosylation activity of potato D-enzyme on amylopectin
Citation
Takaha, T and Yanase, M and Takata, H and Okada, S and Smith, SM, Cyclic glucans produced by the intramolecular transglycosylation activity of potato D-enzyme on amylopectin, Biochemical and Biophysical Research Communications, 247, (2) pp. 493-497. ISSN 0006-291X (1998) [Refereed Article]
Copyright Statement
Copyright 1998 Academic Press
DOI: doi:10.1006/bbrc.1998.8817
Abstract
Potato D-enzyme catalyses an intramolecular transglycosylation reaction on amylose to produce cycloamylose, a novel cyclic α-1,4 glucan. To determine if a similar activity could be observed with a high molecular weight branched substrate, recombinant potato D-enzyme was incubated with amylopectin. The substrate was converted into two fractions of lower molecular mass. Fraction I comprised 15% cyclic molecules of which the majority contained both α-1,4 and α-1,6 links. These were shown to be branched molecules with branches shorter than those in amylopectin. Fraction II comprised 80% cyclic molecules of which the majority contained only α-1,4 links (cycloamylose). Since fraction II appeared before fraction I, we propose that D-enzyme first catalysed the cyclisation of the outer side chains of amylopectin and then the cyclisation of inner chains to produce branched clusters. These results demonstrate that D-enzyme can catalyse the transfer of branched glucans, and suggest novel ways in which it may participate in starch metabolism in plants.
Item Details
Item Type: | Refereed Article |
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Keywords: | cyclic glucans, intramolecular transglycosylation, potato d-enzyme, amylopectin, 4-a-glucanotransferase |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Smith, SM (Professor Steven Smith) |
ID Code: | 101570 |
Year Published: | 1998 |
Web of Science® Times Cited: | 38 |
Deposited By: | Plant Science |
Deposited On: | 2015-06-26 |
Last Modified: | 2015-09-21 |
Downloads: | 0 |
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