eCite Digital Repository
Transformation of chilli pepper (Capsicum frutescens) with a phenylalanine ammonia-lyase gene
Citation
Yamakawa, T and Sekiguchi, S and Kodama, T and Smith, SM and Yeoman, MM, Transformation of chilli pepper (Capsicum frutescens) with a phenylalanine ammonia-lyase gene, Plant Biotechnology, 15, (4) pp. 189-193. ISSN 1342-4580 (1998) [Refereed Article]
Copyright Statement
Copyright 1998 Japanese Society for Plant Cell and Molecular Biology
DOI: doi:10.5511/plantbiotechnology.15.189
Abstract
Hypocotyl explants of chilli pepper (Capsicum frutescens) were inoculated with Agrobacterium rhizogenes strain A13 harbouring the binary vector pBI121, which contains the CaMV 35S promoter linked to the GUS reporter gene. After 4 weeks of culture, the hairy roots produced were transferred onto Schenk and Hildebrandt (SH) medium supplemented with 250mgl-1 Cefotaxime. Subsequently they were selected in liquid SH medium containing 20mgl-1 kanamycin. When treated with X-glucuronide, vascular and root tip tissues were predominantly stained. A phenylalanine ammonia-lyase (PAL) cDNA from parsley (Petroselinum) was subsequently linked to the 35S promoter and transferred into hairy roots using the same A. rhizogenes strain. Successful transformation was confirmed by Southern hybridisation. Hairy roots containing the PAL transgene showed different PAL activity, slow growth and altered morphology.
Item Details
Item Type: | Refereed Article |
---|---|
Keywords: | capsicum fretescens, phenylalanine ammonia-lyase gene |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Smith, SM (Professor Steven Smith) |
ID Code: | 101568 |
Year Published: | 1998 |
Deposited By: | Plant Science |
Deposited On: | 2015-06-26 |
Last Modified: | 2015-09-21 |
Downloads: | 0 |
Repository Staff Only: item control page