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Transformation of chilli pepper (Capsicum frutescens) with a phenylalanine ammonia-lyase gene

Citation

Yamakawa, T and Sekiguchi, S and Kodama, T and Smith, SM and Yeoman, MM, Transformation of chilli pepper (Capsicum frutescens) with a phenylalanine ammonia-lyase gene, Plant Biotechnology, 15, (4) pp. 189-193. ISSN 1342-4580 (1998) [Refereed Article]

Copyright Statement

Copyright 1998 Japanese Society for Plant Cell and Molecular Biology

DOI: doi:10.5511/plantbiotechnology.15.189

Abstract

Hypocotyl explants of chilli pepper (Capsicum frutescens) were inoculated with Agrobacterium rhizogenes strain A13 harbouring the binary vector pBI121, which contains the CaMV 35S promoter linked to the GUS reporter gene. After 4 weeks of culture, the hairy roots produced were transferred onto Schenk and Hildebrandt (SH) medium supplemented with 250mgl-1 Cefotaxime. Subsequently they were selected in liquid SH medium containing 20mgl-1 kanamycin. When treated with X-glucuronide, vascular and root tip tissues were predominantly stained. A phenylalanine ammonia-lyase (PAL) cDNA from parsley (Petroselinum) was subsequently linked to the 35S promoter and transferred into hairy roots using the same A. rhizogenes strain. Successful transformation was confirmed by Southern hybridisation. Hairy roots containing the PAL transgene showed different PAL activity, slow growth and altered morphology.

Item Details

Item Type:Refereed Article
Keywords:capsicum fretescens, phenylalanine ammonia-lyase gene
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Smith, SM (Professor Steven Smith)
ID Code:101568
Year Published:1998
Deposited By:Plant Science
Deposited On:2015-06-26
Last Modified:2015-09-21
Downloads:0

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