eCite Digital Repository
The role of D-enzyme in starch metabolism in plants
Citation
Takaha, T and Okada, S and Smith, SM, The role of D-enzyme in starch metabolism in plants, Journal of Applied Glycoscience, 45, (2) pp. 169-175. ISSN 1344-7882 (1998) [Refereed Article]
Copyright Statement
Copyright 1998 The Japanese Society of Applied Glycoscience
DOI: doi:10.11541/jag1994.45.169
Abstract
D-enzyme (EC 2.4.1.25) is believed to be involved in starch metabolism, but the function in vivo is not known. In order to investigate the role of D-enzyme, several approaches have been undertaken. A biochemical analysis of the purified potato D-enzyme suggested that high molecular weight starch (amylose and amylopectin) can serve as donor and acceptor, and very long α-1, 4-glucans or even highly branched glucans can be transferred by the enzyme. Transgenic potato plants with dramatically reduced D-enzyme activity were obtained by introducing sense and antisense D-enzyme cDNA sequences with the appropriate promoter sequence. The tubers from these plants sprouted later and the growth of sprouts was slower than the wild type. However, no significant difference was found in starch produced in tubers, either in its quantity or quality. From these results, the possible role of Denzyme in starch metabolism is discussed.
Item Details
Item Type: | Refereed Article |
---|---|
Keywords: | starch metabolism, D-enzyme |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Smith, SM (Professor Steven Smith) |
ID Code: | 101567 |
Year Published: | 1998 |
Deposited By: | Plant Science |
Deposited On: | 2015-06-26 |
Last Modified: | 2015-09-21 |
Downloads: | 0 |
Repository Staff Only: item control page