D-enzyme (EC 2.4.1.25) is believed to be involved in starch metabolism, but the function in vivo is not known. In order to investigate the role of D-enzyme, several approaches have been undertaken. A biochemical analysis of the purified potato D-enzyme suggested that high molecular weight starch (amylose and amylopectin) can serve as donor and acceptor, and very long α-1, 4-glucans or even highly branched glucans can be transferred by the enzyme. Transgenic potato plants with dramatically reduced D-enzyme activity were obtained by introducing sense and antisense D-enzyme cDNA sequences with the appropriate promoter sequence. The tubers from these plants sprouted later and the growth of sprouts was slower than the wild type. However, no significant difference was found in starch produced in tubers, either in its quantity or quality. From these results, the possible role of Denzyme in starch metabolism is discussed.

Item Type:	Refereed Article
Keywords:	starch metabolism, D-enzyme
Research Division:	Biological Sciences
Research Group:	Plant Biology
Research Field:	Plant Physiology
Objective Division:	Expanding Knowledge
Objective Group:	Expanding Knowledge
Objective Field:	Expanding Knowledge in the Biological Sciences
UTAS Author:	Smith, SM (Professor Steven Smith)
ID Code:	101567
Year Published:	1998
Deposited By:	Plant Science
Deposited On:	2015-06-26
Last Modified:	2015-09-21
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