eCite Digital Repository
Sugar effect on the malate synthase and isocitrate lyase gene expression at the level of mRNA stability
Citation
Ismail, I and Smith, SM, Sugar effect on the malate synthase and isocitrate lyase gene expression at the level of mRNA stability, Asia-Pacific Journal of Molecular Biology and Biotechnology, 8, (1) pp. 47-56. ISSN 0128-7451 (2000) [Refereed Article]
Abstract
Regulation of malate synthase (MS) and isocitrate lyase (ICL) gene at the posttranscriptional level was studied using actinomycin D, a transcription inhibitor. Detached roots which contained an abundance of NIS and ICL transcripts due to starvation for 4 days, showed a decrease in mRNA amount of about 50% after 3 h incubation in the presence of 10 ug/mI actinomycin D. In contrast, when sucrose was added together with actinomycin D, MS and ICL mRNA decreased to a much lower level within 3 h. Therefore, NIS and ICL half lives appear much shorter in the presence of sugar. Similar results were obtained with detached cotyledons. Confirmation that actinomycin D had effectively stopped transcription was obtained by showing that it prevented light induced expression of hydroxypyruvate reductase and rubisco genes in cotyledons. Thus, NIS and ICL genes appear to be regulated by sugars at the levels of transcription and mRNA stability.
Item Details
Item Type: | Refereed Article |
---|---|
Keywords: | malate synthase, isocitrate lyase, mRNA |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Smith, SM (Professor Steven Smith) |
ID Code: | 101559 |
Year Published: | 2000 |
Deposited By: | Plant Science |
Deposited On: | 2015-06-25 |
Last Modified: | 2015-07-08 |
Downloads: | 0 |
Repository Staff Only: item control page