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Using gene knockouts to investigate plant metabolism


Thorneycroft, D and Sherson, SM and Smith, SM, Using gene knockouts to investigate plant metabolism, Journal of Experimental Botany, 52, (361) pp. 1593-1601. ISSN 0022-0957 (2001) [Refereed Article]

Copyright Statement

Copyright 2001 Society for Experimental Biology

DOI: doi:10.1093/jexbot/52.361.1593


Arabidopsis functional genomics resources now make the isolation of knockout mutants in any gene of choice both realistic and increasingly straightforward. Coupled with the completion of the genome sequence, this reverse genetics approach provides a platform facilitating dramatic progress in our understanding of fundamental aspects of plant metabolism. Recent experience shows that knockouts of genes encoding enzymes of primary metabolism can produce mutants with clear and sometimes unexpected phenotypes. They can provide new information about old pathways. Specific functions for individual members of multigene families can be revealed. Knockouts of enzymes of undefined function can lead to the discovery of those functions, and the analysis of enzymes which have previously never been studied at the biochemical level offers the potential to reveal new pathways of plant metabolism. Furthermore, the mutants isolated provide the starting point for genetic modification experiments to determine exactly how metabolism fuels growth and development, so providing a rational basis for the future modification of plant productivity.

Item Details

Item Type:Refereed Article
Keywords:Arabidopsis thaliana, insertional mutagenesis, gene knockouts, reverse genetics, plant metabolism, plant growth
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Smith, SM (Professor Steven Smith)
ID Code:101555
Year Published:2001
Web of Science® Times Cited:32
Deposited By:Plant Science
Deposited On:2015-06-25
Last Modified:2015-09-21

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