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Using gene knockouts to investigate plant metabolism
Citation
Thorneycroft, D and Sherson, SM and Smith, SM, Using gene knockouts to investigate plant metabolism, Journal of Experimental Botany, 52, (361) pp. 1593-1601. ISSN 0022-0957 (2001) [Refereed Article]
Copyright Statement
Copyright 2001 Society for Experimental Biology
DOI: doi:10.1093/jexbot/52.361.1593
Abstract
Arabidopsis functional genomics resources now make the isolation of knockout mutants in any gene of choice both realistic and increasingly
straightforward. Coupled with the completion of the genome sequence, this reverse genetics approach provides a platform facilitating
dramatic progress in our understanding of fundamental aspects of plant metabolism. Recent experience shows that knockouts
of genes encoding enzymes of primary metabolism can produce mutants with clear and sometimes unexpected phenotypes. They can
provide new information about old pathways. Specific functions for individual members of multigene families can be revealed.
Knockouts of enzymes of undefined function can lead to the discovery of those functions, and the analysis of enzymes which
have previously never been studied at the biochemical level offers the potential to reveal new pathways of plant metabolism.
Furthermore, the mutants isolated provide the starting point for genetic modification experiments to determine exactly how
metabolism fuels growth and development, so providing a rational basis for the future modification of plant productivity.
Item Details
Item Type: | Refereed Article |
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Keywords: | Arabidopsis thaliana, insertional mutagenesis, gene knockouts, reverse genetics, plant metabolism, plant growth |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Smith, SM (Professor Steven Smith) |
ID Code: | 101555 |
Year Published: | 2001 |
Web of Science® Times Cited: | 32 |
Deposited By: | Plant Science |
Deposited On: | 2015-06-25 |
Last Modified: | 2015-09-21 |
Downloads: | 0 |
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