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Cyclization reaction catalyzed by glycogen debranching enzyme (EC 2.4.1.25/EC 3.2.1.33) and its potential for cycloamylose production
Citation
Yanase, M and Takata, H and Takaha, T and Kuriki, T and Smith, SM and Okada, S, Cyclization reaction catalyzed by glycogen debranching enzyme (EC 2.4.1.25/EC 3.2.1.33) and its potential for cycloamylose production, Applied and Environmental Microbiology, 68, (9) pp. 4233-4239. ISSN 0099-2240 (2002) [Refereed Article]
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Copyright Statement
Copyright 2002 American Society for Microbiology
DOI: doi:10.1128/AEM.68.9.4233-4239.2002
Abstract
Glycogen debranching enzyme (GDE) has 4-a-glucanotransferase and amylo-1,6-glucosidase activities in the
single polypeptide chain. We analyzed the detailed action profile of GDE from Saccharomyces cerevisiae on
amylose and tested whether GDE catalyzes cyclization of amylose. GDE treatment resulted in a rapid reduction
of absorbance of iodine-amylose complex and the accumulation of a product that was resistant to an exoamylase
(glucoamylase [GA]) but was degraded by an endo-type a-amylase to glucose and maltose. These
results indicated that GDE catalyzed cyclization of amylose to produce cyclic a-1,4 glucan (cycloamylose). The
formation of cycloamylose was confirmed by high-performance anion-exchange chromatography, and the size
was shown to range from a degree of polymerization of 11 to a degree of polymerization around 50. The
minimum size and the size distribution of cycloamylose were different from those of cycloamylose produced by
other 4-a-glucanotransferases. GDE also efficiently produced cycloamylose even from the branched glucan
substrate, starch, demonstrating its potential for industrial production of cycloamylose.
Item Details
Item Type: | Refereed Article |
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Keywords: | glycogen debranching enzyme;cycloamylose production |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Smith, SM (Professor Steven Smith) |
ID Code: | 101550 |
Year Published: | 2002 |
Web of Science® Times Cited: | 20 |
Deposited By: | Plant Science |
Deposited On: | 2015-06-25 |
Last Modified: | 2022-09-02 |
Downloads: | 222 View Download Statistics |
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