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Plastidial α-glucan phosphorylase is not required for starch degradation in Arabidopsis leaves but has a role in the tolerance of abiotic stress

Citation

Zeeman, SC and Thorneycroft, D and Schupp, N and Chapple, A and Weck, M and Dunstan, H and Haldimann, P and Bechtold, N and Smith, AM and Smith, SM, Plastidial α-glucan phosphorylase is not required for starch degradation in Arabidopsis leaves but has a role in the tolerance of abiotic stress, Plant Physiology, 135, (2) pp. 849-858. ISSN 0032-0889 (2004) [Refereed Article]

Copyright Statement

Copyright 2004 American Society of Plant Biologists

DOI: doi:10.​1104/​pp.​103.​032631

Abstract

To study the role of the plastidial α-glucan phosphorylase in starch metabolism in the leaves of Arabidopsis, two independent mutant lines containing T-DNA insertions within the phosphorylase gene were identified. Both insertions eliminate the activity of the plastidial α-glucan phosphorylase. Measurement of other enzymes of starch metabolism reveals only minor changes compared with the wild type. The loss of plastidial α-glucan phosphorylase does not cause a significant change in the total accumulation of starch during the day or its remobilization at night. Starch structure and composition are unaltered. However, mutant plants display lesions on their leaves that are not seen on wild-type plants, and mesophyll cells bordering the lesions accumulate high levels of starch. Lesion formation is abolished by growing plants under 100% humidity in still air, but subsequent transfer to circulating air with lower humidity causes extensive wilting in the mutant leaves. Wilted sectors die, causing large lesions that are bordered by starch-accumulating cells. Similar lesions are caused by the application of acute salt stress to mature plants. We conclude that plastidial phosphorylase is not required for the degradation of starch, but that it plays a role in the capacity of the leaf lamina to endure a transient water deficit.

Item Details

Item Type:Refereed Article
Keywords:starch degradation, arabidopsis, stress tolerance, alpha-glucan
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Smith, SM (Professor Steven Smith)
ID Code:101544
Year Published:2004
Web of Science® Times Cited:134
Deposited By:Plant Science
Deposited On:2015-06-25
Last Modified:2015-09-21
Downloads:0

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