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Plastidial α-glucan phosphorylase is not required for starch degradation in Arabidopsis leaves but has a role in the tolerance of abiotic stress
Citation
Zeeman, SC and Thorneycroft, D and Schupp, N and Chapple, A and Weck, M and Dunstan, H and Haldimann, P and Bechtold, N and Smith, AM and Smith, SM, Plastidial α-glucan phosphorylase is not required for starch degradation in Arabidopsis leaves but has a role in the tolerance of abiotic stress, Plant Physiology, 135, (2) pp. 849-858. ISSN 0032-0889 (2004) [Refereed Article]
Copyright Statement
Copyright 2004 American Society of Plant Biologists
DOI: doi:10.1104/pp.103.032631
Abstract
To study the role of the plastidial α-glucan phosphorylase in starch metabolism in the leaves of Arabidopsis, two independent mutant lines containing T-DNA insertions
within the phosphorylase gene were identified. Both insertions eliminate the activity of the plastidial α-glucan phosphorylase. Measurement of other enzymes of starch metabolism reveals only minor changes compared with the wild
type. The loss of plastidial α-glucan phosphorylase does not cause a significant change in the total accumulation of starch during the day or its remobilization
at night. Starch structure and composition are unaltered. However, mutant plants display lesions on their leaves that are
not seen on wild-type plants, and mesophyll cells bordering the lesions accumulate high levels of starch. Lesion formation
is abolished by growing plants under 100% humidity in still air, but subsequent transfer to circulating air with lower humidity
causes extensive wilting in the mutant leaves. Wilted sectors die, causing large lesions that are bordered by starch-accumulating
cells. Similar lesions are caused by the application of acute salt stress to mature plants. We conclude that plastidial phosphorylase
is not required for the degradation of starch, but that it plays a role in the capacity of the leaf lamina to endure a transient
water deficit.
Item Details
Item Type: | Refereed Article |
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Keywords: | starch degradation, arabidopsis, stress tolerance, alpha-glucan |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Smith, SM (Professor Steven Smith) |
ID Code: | 101544 |
Year Published: | 2004 |
Web of Science® Times Cited: | 176 |
Deposited By: | Plant Science |
Deposited On: | 2015-06-25 |
Last Modified: | 2015-09-21 |
Downloads: | 0 |
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