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Arabidopsis AtcwINV3 and 6 are not invertases but are fructan exohydrolases (FEHs) with different substrate specificities
Citation
de Coninck, B and le Roy, K and Francis, I and Clerens, S and Vergauwen, R and Halliday, AM and Smith, SM and van Laere, A and van den Ende, W, Arabidopsis AtcwINV3 and 6 are not invertases but are fructan exohydrolases (FEHs) with different substrate specificities, Plant, Cell and Environment, 28, (4) pp. 432-443. ISSN 0140-7791 (2005) [Refereed Article]
Copyright Statement
© 2005 Blackwell Publishing Ltd
DOI: doi:10.1111/j.1365-3040.2004.01281.x
Abstract
The genome of Arabidopsis thaliana contains six putative cell-wall type invertase genes (AtcwINV1-6). Heterologous expression of AtcwINV1, 3 and 6 cDNAs in Pichia pastoris revealed that the enzymes encoded by AtcwINV3 and 6 did not show invertase activity. Instead, AtcwINV3 is a 6-FEH and AtcwINV6 is a fructan exohydrolase (FEH) that can degrade both inulin and levan-type fructans. For AtcwINV6 it is proposed to use the term (6&1) FEH. In contrast, AtcwINV1 is a typical invertase. FEH activity was also detected in crude extracts of different parts of Arabidopsis. To verify that the FEH activity of AtcwINV3 and 6 were not artefacts of the heterologous expression system, the protein corresponding to AtcwINV3 was isolated from whole Arabidopsis plants and indeed showed only 6-FEH activity and no invertase activity. Although no fructans can be detected in Arabidopsis plants, it is shown that kestoses (trimers) can be synthesized in crude leaf extracts. The putative physiological significance of FEH in so-called non-fructan plants is discussed.
Item Details
Item Type: | Refereed Article |
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Keywords: | Pichia pastoris, Arabidopsis, cell-wall inver-tase, defence, inulin, levan |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Smith, SM (Professor Steven Smith) |
ID Code: | 101521 |
Year Published: | 2005 |
Web of Science® Times Cited: | 103 |
Deposited By: | Plant Science |
Deposited On: | 2015-06-25 |
Last Modified: | 2015-09-27 |
Downloads: | 0 |
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