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Arabidopsis peroxisomal citrate synthase is required for fatty acid respiration and seed germination
Citation
Pracharoenwattana, I and Cornah, JE and Smith, SM, Arabidopsis peroxisomal citrate synthase is required for fatty acid respiration and seed germination, Plant Cell, 17, (7) pp. 2037-2048. ISSN 1040-4651 (2005) [Refereed Article]
Copyright Statement
Copyright 2005 American Society of Plant Biologists
DOI: doi:10.1105/tpc.105.031856
Abstract
We tested the hypothesis that peroxisomal citrate synthase (CSY) is required for carbon transfer from peroxisomes to
mitochondria during respiration of triacylglycerol in Arabidopsis thaliana seedlings. Twogenes encoding peroxisomal CSYare
expressed in Arabidopsis seedlings, and seeds from plants with both CSY genes disrupted were dormant and did not
metabolize triacylglycerol. Germination was achieved by removing the seed coat and supplying sucrose, but the seedlings still
did not use triacylglycerol. The mutant seedlings were resistant to 2,4-dichlorophenoxybutyric acid, indicating a block in
peroxisomal b-oxidation, and were unable to develop further after transfer to soil. The mutant phenotype was complemented
with acDNAencodingCSYwith either its native peroxisomal targeting sequence (PTS2) or a heterologous PTS1sequence from
pumpkin (Cucurbita pepo) malate synthase. These results suggest that peroxisomal CSY in Arabidopsis is not only a key
enzyme of the glyoxylate cycle but also catalyzes an essential step in the respiration of fatty acids. We conclude that citrate is
exported from the peroxisome during fatty acid respiration, whereas in yeast, acetylcarnitine is exported.
Item Details
Item Type: | Refereed Article |
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Keywords: | arabidopsis thaliana, peroxisomal, fatty acid respiration |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Smith, SM (Professor Steven Smith) |
ID Code: | 101518 |
Year Published: | 2005 |
Web of Science® Times Cited: | 156 |
Deposited By: | Plant Science |
Deposited On: | 2015-06-25 |
Last Modified: | 2017-06-06 |
Downloads: | 0 |
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