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Arabidopsis peroxisomal malate dehydrogenase functions in β-oxidation but not in the glyoxylate cycle
Citation
Pracharoenwattana, I and Cornah, JE and Smith, SM, Arabidopsis peroxisomal malate dehydrogenase functions in β-oxidation but not in the glyoxylate cycle, The Plant Journal, 50, (3) pp. 381-390. ISSN 0960-7412 (2007) [Refereed Article]
Copyright Statement
Copyright 2007 The Authors
DOI: doi:10.1111/j.1365-313X.2007.03055.x
Abstract
The aim was to determine the function of peroxisomal NAD+-malate dehydrogenase (PMDH) in fatty acid β-oxidation and the glyoxylate cycle in Arabidopsis. Seeds in which both PMDH genes are disrupted by T-DNA insertions germinate, but seedling establishment is dependent on exogenous sugar. Mutant seedlings mobilize their triacylglycerol very slowly and growth is insensitive to 2,4-dichlorophenoxybutyric acid. Thus mutant seedlings are severely impaired in β-oxidation, even though microarray analysis shows that β-oxidation genes are expressed normally. The mutant phenotype was complemented by expression of a cDNA encoding PMDH with either its native peroxisome targeting signal-2 (PTS2) targeting sequence or a heterologous PTS1 sequence. In contrast to the block in β-oxidation in mutant seedlings, [14C]acetate is readily metabolized into sugars and organic acids, thereby demonstrating normal activity of the glyoxylate cycle. We conclude that PMDH serves to reoxidize NADH produced from fatty acid β-oxidation and does not participate directly in the glyoxylate cycle.
Item Details
Item Type: | Refereed Article |
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Keywords: | peroxisome, malate dehydrogenase, b-oxidation, glyoxylate cycle, fatty acids, Arabidopsis |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Smith, SM (Professor Steven Smith) |
ID Code: | 101509 |
Year Published: | 2007 |
Web of Science® Times Cited: | 113 |
Deposited By: | Plant Science |
Deposited On: | 2015-06-25 |
Last Modified: | 2015-09-24 |
Downloads: | 0 |
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