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The Arabidopsis 3-ketoacyl-CoA thiolase-2 (kat2-1) mutant exhibits increased flowering but reduced reproductive success

Citation

Footitt, S and Cornah, JE and Pracharoenwattana, I and Bryce, JH and Smith, SM, The Arabidopsis 3-ketoacyl-CoA thiolase-2 (kat2-1) mutant exhibits increased flowering but reduced reproductive success, Journal of Experimental Botany, 58, (11) pp. 2959-2968. ISSN 0022-0957 (2007) [Refereed Article]

Copyright Statement

Copyright 2007 The Author

DOI: doi:10.1093/jxb/erm146

Abstract

The enzyme 3-ketoacyl-CoA thiolase (KAT) (EC 2.3.1.16) catalyses a key step in fatty acid β-oxidation. In Arabidopsis thaliana, expression of the KAT2 gene is known to be required for the efficient mobilization of triacylglycerol during germination and seedling establishment. Here, data from the Arabidopsis kat2-1 mutant are presented, showing that perturbation of β-oxidation also affects vegetative growth and reproductive success. In the wild type, the KAT2 protein was detected in all organs tested. In the kat2-1 mutant, rosette leaf area and dry weight, but not leaf number, were greatly increased relative to wild type. Global proliferative arrest of flowering was delayed, resulting in increased silique production in kat2-1 plants. However, total silique dry weight was not increased. kat2-1 siliques were smaller and had a reduced seed number caused by increased ovule abortion. In kat2-1 ovules, carbon flow into sugars via gluconeogeneis and respiration were both reduced in comparison to the wild type. In conclusion, these data indicate that a functional β-oxidation pathway is required to maintain the balance between silique development and the continued initiation of floral meristems.

Item Details

Item Type:Refereed Article
Keywords:Arabidopsis, beta-oxidation, embryogenesis, flowering, lipid mobilization, peroxisome, respiration.
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Smith, SM (Professor Steven Smith)
ID Code:101507
Year Published:2007
Web of Science® Times Cited:30
Deposited By:Plant Science
Deposited On:2015-06-25
Last Modified:2015-09-24
Downloads:0

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