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The metabolome of Chlamydomonas reinhardtii following induction of anaerobic H2 production by sulfur depletion
Citation
Matthew, T and Zhou, W and Rupprecht, J and Lim, L and Thomas-Hall, SR and Doebbe, A and Kruse, O and Hankamer, B and Marx, UC and Smith, SM and Schenk, PM, The metabolome of Chlamydomonas reinhardtii following induction of anaerobic H2 production by sulfur depletion, Journal of Biological Chemistry, 284, (35) pp. 23415-23425. ISSN 0021-9258 (2009) [Refereed Article]
Copyright Statement
© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.
DOI: doi:10.1074/jbc.M109.003541
Abstract
The metabolome of the model species Chlamydomonas reinhardtii has been analyzed during 120 h of sulfur depletion to induce anaerobic hydrogen (H2) production, using NMR spectroscopy, gas chromatography coupled to mass spectrometry, and TLC. The results indicate that these unicellular green algae consume freshly supplied acetate in the medium to accumulate energy reserves during the first 24 h of sulfur depletion. In addition to the previously reported accumulation of starch, large amounts of triacylglycerides were deposited in the cells. During the early 24- to 72-h time period fermentative energy metabolism lowered the pH, H2 was produced, and amino acid levels generally increased. In the final phase from 72 to 120 h, metabolism slowed down leading to a stabilization of pH, even though some starch and most triacylglycerides remained. We conclude that H2 production does not slow down due to depletion of energy reserves but rather due to loss of essential functions resulting from sulfur depletion or due to a build-up of the toxic fermentative products formate and ethanol.
Item Details
Item Type: | Refereed Article |
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Keywords: | metabolome, chlamydomonas reinhardtii, anaerobic hydrogen production, green algae |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Smith, SM (Professor Steven Smith) |
ID Code: | 101475 |
Year Published: | 2009 |
Web of Science® Times Cited: | 111 |
Deposited By: | Plant Science |
Deposited On: | 2015-06-24 |
Last Modified: | 2016-09-30 |
Downloads: | 0 |
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