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The nucleotidase/phosphatase SAL1 is a negative regulator of drought tolerance in Arabidopsis

Citation

Wilson, PB and Estavillo, GM and Field, KJ and Pornsiriwong, W and Carroll, AJ and Howell, KA and Woo, NS and Lake, JA and Smith, SM and Millar, AH and von Caemmerer, S and Pogson, BJ, The nucleotidase/phosphatase SAL1 is a negative regulator of drought tolerance in Arabidopsis, The Plant Journal, 58, (2) pp. 299-317. ISSN 0960-7412 (2009) [Refereed Article]

Copyright Statement

2009 The Authors Journal compilation 2009 Blackwell Publishing Ltd

DOI: doi:10.1111/j.1365-313X.2008.03780.x

Abstract

An Arabidopsis thaliana drought-tolerant mutant, altered expression of APX2 (alx8), has constitutively increased abscisic acid (ABA) content, increased expression of genes responsive to high light stress and is reported to be drought tolerant. We have identified alx8 as a mutation in SAL1, an enzyme that can dephosphorylate dinucleotide phosphates or inositol phosphates. Previously identified mutations in SAL1, including fiery (fry1-1), were reported as being more sensitive to drought imposed by detachment of rosettes. Here we demonstrate that alx8, fry1-1 and a T-DNA insertional knockout allele all have markedly increased resistance to drought when water is withheld from soil-grown intact plants. Microarray analysis revealed constitutively altered expression of more than 1800 genes in both alx8 and fry1-1. The up-regulated genes included some characterized stress response genes, but few are inducible by ABA. Metabolomic analysis revealed that both mutants exhibit a similar, dramatic reprogramming of metabolism, including increased levels of the polyamine putrescine implicated in stress tolerance, and the accumulation of a number of unknown, potential osmoprotectant carbohydrate derivatives. Under well-watered conditions, there was no substantial difference between alx8 and Col-0 in biomass at maturity; plant water use efficiency (WUE) as measured by carbon isotope discrimination; or stomatal index, morphology or aperture. Thus, SAL1 acts as a negative regulator of predominantly ABA-independent and also ABA-dependent stress response pathways, such that its inactivation results in altered osmoprotectants, higher leaf relative water content and maintenance of viable tissues during prolonged water stress.

Item Details

Item Type:Refereed Article
Keywords:drought, water use efficiency, SAL1, Arabidopsis, signal transduction, phosphoinositides (PI). Introduction Abiotic
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Smith, SM (Professor Steven Smith)
ID Code:101474
Year Published:2009
Web of Science® Times Cited:100
Deposited By:Plant Science
Deposited On:2015-06-24
Last Modified:2015-09-22
Downloads:0

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