101437 - the structure of the karrikin.PDF (666.96 kB)
The structure of the karrikin-insensitive protein (KAI2) in Arabidopsis thaliana
journal contribution
posted on 2023-05-18, 11:11 authored by Bythell-Douglas, R, Waters, MT, Scaffidi, A, Flematti, GR, Steven SmithSteven Smith, Bond, CSKARRIKIN INSENSITIVE 2 (KAI2) is an α/β hydrolase involved in seed germination and seedling development. It is essential for plant responses to karrikins, a class of butenolide compounds derived from burnt plant material that are structurally similar to strigolactone plant hormones. The mechanistic basis for the function of KAI2 in plant development remains unclear. We have determined the crystal structure of Arabidopsis thaliana KAI2 in space groups P21 21 21 (a = 63.57 Å, b = 66.26 Å, c = 78.25 Å) and P21 (a = 50.20 Å, b = 56.04 Å, c = 52.43 Å, β = 116.12°) to 1.55 and 2.11 Å respectively. The catalytic residues are positioned within a large hydrophobic pocket similar to that of DAD2, a protein required for strigolactone response in Petunia hybrida. KAI2 possesses a second solvent-accessible pocket, adjacent to the active site cavity, which offers the possibility of allosteric regulation. The structure of KAI2 is consistent with its designation as a serine hydrolase, as well as previous data implicating the protein in karrikin and strigolactone signalling.
History
Publication title
PLoS OneVolume
8Article number
e54758Number
e54758Pagination
1-8ISSN
1932-6203Department/School
School of Natural SciencesPublisher
Public Library of SciencePlace of publication
United States of AmericaRights statement
Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) http://creativecommons.org/licenses/by/4.0/Repository Status
- Open