eCite Digital Repository
The structure of the karrikin-insensitive protein (KAI2) in Arabidopsis thaliana
Citation
Bythell-Douglas, R and Waters, MT and Scaffidi, A and Flematti, GR and Smith, SM and Bond, CS, The structure of the karrikin-insensitive protein (KAI2) in Arabidopsis thaliana, PLoS One, 8, (1) Article e54758. ISSN 1932-6203 (2013) [Refereed Article]
![]() | PDF 667Kb |
Copyright Statement
Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) http://creativecommons.org/licenses/by/4.0/
DOI: doi:10.1371/journal.pone.0054758
Abstract
KARRIKIN INSENSITIVE 2 (KAI2) is an α/β hydrolase involved in seed germination and seedling development. It is essential for plant responses to karrikins, a class of butenolide compounds derived from burnt plant material that are structurally similar to strigolactone plant hormones. The mechanistic basis for the function of KAI2 in plant development remains unclear. We have determined the crystal structure of Arabidopsis thaliana KAI2 in space groups P21 21 21 (a = 63.57 Å, b = 66.26 Å, c = 78.25 Å) and P21 (a = 50.20 Å, b = 56.04 Å, c = 52.43 Å, β = 116.12°) to 1.55 and 2.11 Å respectively. The catalytic residues are positioned within a large hydrophobic pocket similar to that of DAD2, a protein required for strigolactone response in Petunia hybrida. KAI2 possesses a second solvent-accessible pocket, adjacent to the active site cavity, which offers the possibility of allosteric regulation. The structure of KAI2 is consistent with its designation as a serine hydrolase, as well as previous data implicating the protein in karrikin and strigolactone signalling.
Item Details
Item Type: | Refereed Article |
---|---|
Keywords: | arabidopsis thaliana, karrikin, seed germination, seedling development, butenolide |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Smith, SM (Professor Steven Smith) |
ID Code: | 101437 |
Year Published: | 2013 |
Web of Science® Times Cited: | 42 |
Deposited By: | Plant Science |
Deposited On: | 2015-06-24 |
Last Modified: | 2018-04-11 |
Downloads: | 63 View Download Statistics |
Repository Staff Only: item control page