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Acclimation-induced changes in cell membrane composition and influence on cryotolerance of in vitro shoots of native plant species

Citation

Funnekotter, B and Kaczmarczyk, A and Turner, SR and Bunn, E and Zhou, W and Smith, S and Flematti, G and Mancera, RL, Acclimation-induced changes in cell membrane composition and influence on cryotolerance of in vitro shoots of native plant species, Plant Cell, Tissue and Organ Culture, 114, (1) pp. 83-96. ISSN 0167-6857 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 Springer Science+Business Media Dordrecht

DOI: doi:10.1007/s11240-013-0308-0

Abstract

Cell membranes are the primary sites of cryopreservation injury and measuring changes to membrane composition arising from cold acclimation may assist with providing a rationale for optimising cryopreservation methods. Shoot tips from two south-west Western Australian species, Grevillea scapigera and Loxocarya cinerea, and Arabidopsis thaliana (reference species) were subjected to cryopreservation using the droplet vitrification protocol. Two pre-conditioning regimes involving a constant temperature (23C, CT with a 12h light/dark cycle) or an alternating temperature (AT) regime (20/10C with a 12h light/dark cycle) were compared. Soluble sugars, sterols and phospholipids present in the shoot tips were analysed. Use of AT pre-conditioning (acclimation) resulted in a modest decrease in cryotolerance in A. thaliana, increased cryotolerance in G. scapigera, and increased survival in the non-frozen control explants of L. cinerea in comparison to CT pre-conditioning. Increased cryotolerance was accompanied by a higher total sugar sterol and phospholipid content, as well as an increase in strong hydrating phospholipid classes such as phosphatidylcholine. The double bond index of bound fatty acyl chains of phospholipids was greater after AT pre-conditioning, mostly due to a higher amount of monoenes in A. thaliana and trienes in G. scapigera and L. cinerea. These findings suggest that AT pre-conditioning treatments for in vitro plants can have a positive influence on cryotolerance for some plant species and this may be related to observed changes in the overall composition of cell membranes. However, alternative factors (e.g. oxidative stress) may be equally important with other species (e.g. L. cinerea).

Item Details

Item Type:Refereed Article
Keywords:Arabidopsis thaliana, cryopreservation, Grevillea scapigera, Loxocarya cinerea, phospholipids, sterols, vitrification
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Smith, S (Professor Steven Smith)
ID Code:101421
Year Published:2013
Web of Science® Times Cited:12
Deposited By:Plant Science
Deposited On:2015-06-23
Last Modified:2017-11-02
Downloads:0

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