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Carlactone-independent seedling morphogenesis in Arabidopsis

journal contribution
posted on 2023-05-18, 11:10 authored by Scaffidi, A, Waters, MT, Ghisalberti, EL, Dixon, KW, Flematti, GR, Steven SmithSteven Smith
Strigolactone hormones are derived from carotenoids via carlactone, and act through the α/β–hydrolase D14 and the F–box protein D3/MAX2 to repress plant shoot branching. While MAX2 is also necessary for normal seedling development, D14 and the known strigolactone biosynthesis genes are not, raising the question of whether endogenous, canonical strigolactones derived from carlactone have a role in seedling morphogenesis. Here, we report the chemical synthesis of the strigolactone precursor carlactone, and show that it represses Arabidopsis shoot branching and influences leaf morphogenesis via a mechanism that is dependent on the cytochrome P450 MAX1. In contrast, both physiologically active Z–carlactone and the non-physiological E isomer exhibit similar weak activity in seedlings, and predominantly signal through D14 rather than its paralogue KAI2, in a MAX2-dependent but MAX1-independent manner. KAI2 is essential for seedling morphogenesis, and hence this early-stage development employs carlactone-independent morphogens for which karrikins from wildfire smoke are specific surrogates. While the commonly employed synthetic strigolactone GR24 acts non-specifically through both D14 and KAI2, carlactone is a specific effector of strigolactone signalling that acts through MAX1 and D14.

History

Publication title

The Plant Journal

Volume

76

Pagination

1-9

ISSN

0960-7412

Department/School

School of Natural Sciences

Publisher

Blackwell Publishing Ltd

Place of publication

9600 Garsington Rd, Oxford, England, Oxon, Ox4 2Dg

Rights statement

Copyright 2013 The Authors, The Plant Journal Copyright 2013 John Wiley & Sons Ltd

Repository Status

  • Restricted

Socio-economic Objectives

Expanding knowledge in the biological sciences

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