Preview

PDF Not available 701Kb

Transcriptional patterns in the expression of genes controlling lipid metabolism in supplemented sheep are currently unknown, thus quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was used to assess the messenger RNA (mRNA) transcription levels and expressions of Aralkylamine N-acetyltransferase (AANAT), Adrenergic beta-3 receptor (ADRB3), B-cell translocation gene 2 (BTG2), and Fatty acid synthase (FASN) genes. The aim was to assess the effect of dietary supplementation with Spirulina, a blue-green cyanobacterial microalga, on the expression of genes controlling fatty acid metabolism in the heart, kidney and liver of prime lambs. In total, 432 heart, kidney and liver samples from 48 purebred and crossbred Merino prime lambs supplemented with low or high levels of Spirulina over a 9-week period were utilized for the study. Both the low and high levels of Spirulina supplementation regimes strongly up-regulated the transcription of all the selected genes. Sire breed and sex of lamb did not influence gene expression patterns; however, significant variations in response to Spirulina supplementation underpin the genetics-nutrition interactions that could be of practical importance for manipulating meat quality in the Australian dual-purpose prime lamb industry for a healthy polyunsaturated fatty acid profile.

Item Type:	Refereed Conference Paper
Keywords:	Arthrospira platensis, gene expression, sheep, heart, kidney, liver
Research Division:	Agricultural, Veterinary and Food Sciences
Research Group:	Animal production
Research Field:	Animal reproduction and breeding
Objective Division:	Animal Production and Animal Primary Products
Objective Group:	Livestock raising
Objective Field:	Sheep for meat
UTAS Author:	Malau-Aduli, AEO (Associate Professor Aduli Malau-Aduli)
UTAS Author:	Kashani, A (Mr Arash Kashani)
ID Code:	101363
Year Published:	2015
Deposited By:	Tasmanian Institute of Agriculture
Deposited On:	2015-06-19
Last Modified:	2016-08-08
Downloads:	0