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Molecular genetics-nutrition interactions in the expression of AANAT, ADRB3, BTG2 and FASN genes in the heart, kidney and liver of Australian lambs supplemented with Spirulina (Arthrospira platensis)
journal contribution
posted on 2023-05-18, 11:08 authored by Malau-Aduli, AEO, Kashani, AQuantitative reverse transcriptase polymerase chain reaction was used to assess the messenger RNA (mRNA) transcription levels and expressions of Aralkylamine N-acetyltransferase, Adrenergic beta-3 receptor, B cell translocation gene 2, and Fatty acid synthase genes. In total, 432 heart, kidney and liver samples from 48 purebred and crossbred Australian prime lambs supplemented with Spirulina (Arthrospira platensis) blue-green cyanobacterial microalgae over a 9-week feeding trial were utilized for fatty acid composition and mRNA extraction. The aim was to assess the effect of dietary Spirulina supplementation on the mRNA expression profiles of genes controlling fatty acid metabolism in the heart, kidney and liver. Fourty-eight weaned lambs belonging to four breeds under the same management conditions were maintained on ryegrass pasture and randomly allocated to three treatment groups that comprised the control and two levels of Spirulina supplementation: Low (100mL/head/day of 1 g Spirulina powder dissolved in 10 mL of water (10% wt:vol)) and High (200 mL/head/day of 2 g of Spirulina dissolved in 10 mL of water (20% wt:vol)). Both the low and high levels of Spirulina supplementation regimes strongly up-regulated the transcription of all the selected genes and increased w-3 and w-6 polyunsaturated fatty acid compositions in the tested organs. Breed and sex did not influence gene expression patterns; however, significant variations in response to Spirulina supplementation underpin the genetics-nutrition interactions that could be of practical importance for manipulating meat quality in the Australian dual-purpose prime lamb industry for a healthy polyunsaturated fatty acid profile.
Funding
Australian Wool Education Trust
History
Publication title
Genes and GenomicsVolume
37Issue
7Pagination
633-644ISSN
1976-9571Department/School
Tasmanian Institute of Agriculture (TIA)Publisher
The Genetics Society of KoreaPlace of publication
Republic of KoreaRights statement
Copyright 2015 The Genetics Society of Korea and Springer-Science and MediaRepository Status
- Restricted