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No effect of NOS inhibition on skeletal muscle glucose uptake during in situ hindlimb contraction in healthy and diabetic Sprague-Dawley rats


Hong, YH and Betik, AC and Premilovac, D and Dwyer, RM and Keske, MA and Rattigan, S and McConell, GK, No effect of NOS inhibition on skeletal muscle glucose uptake during in situ hindlimb contraction in healthy and diabetic Sprague-Dawley rats, American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, 308, (10) pp. R862-R871. ISSN 0363-6119 (2015) [Refereed Article]

Copyright Statement

Copyright 2015 the American Physiological Society

DOI: doi:10.1152/ajpregu.00412.2014


Nitric oxide (NO) has been shown to be involved in skeletal muscle glucose uptake during contraction/exercise, especially in individuals with Type 2 diabetes (T2D). To examine the potential mechanisms, we examined the effect of local NO synthase (NOS) inhibition on muscle glucose uptake and muscle capillary blood flow during contraction in healthy and T2D rats. T2D was induced in Sprague-Dawley rats using a combined high-fat diet (23% fat wt/wt for 4 wk) and low-dose streptozotocin injections (35 mg/kg). Anesthetized animals had one hindlimb stimulated to contract in situ for 30 min (2 Hz, 0.1 ms, 35 V) with the contralateral hindlimb rested. After 10 min, the NOS inhibitor, N(G)-nitro-l-arginine methyl ester (L-NAME; 5 μM) or saline was continuously infused into the femoral artery of the contracting hindlimb until the end of contraction. Surprisingly, there was no increase in skeletal muscle NOS activity during contraction in either group. Local NOS inhibition had no effect on systemic blood pressure or muscle contraction force, but it did cause a significant attenuation of the increase in femoral artery blood flow in control and T2D rats. However, NOS inhibition did not attenuate the increase in muscle capillary recruitment during contraction in these rats. Muscle glucose uptake during contraction was significantly higher in T2D rats compared with controls but, unlike our previous findings in hooded Wistar rats, NOS inhibition had no effect on glucose uptake during contraction. In conclusion, NOS inhibition did not affect muscle glucose uptake during contraction in control or T2D Sprague-Dawley rats, and this may have been because there was no increase in NOS activity during contraction.

Item Details

Item Type:Refereed Article
Keywords:type 2 diabetes, capillary recruitment, contrast-enhanced ultrasound, femoral blood flow, nitric oxide synthase activity
Research Division:Biomedical and Clinical Sciences
Research Group:Clinical sciences
Research Field:Endocrinology
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Premilovac, D (Dr Dino Premilovac)
UTAS Author:Dwyer, RM (Dr Renee Ross)
UTAS Author:Keske, MA (Dr Michelle Keske)
UTAS Author:Rattigan, S (Professor Stephen Rattigan)
ID Code:101349
Year Published:2015
Web of Science® Times Cited:10
Deposited By:Menzies Institute for Medical Research
Deposited On:2015-06-18
Last Modified:2017-11-03

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