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Rice cytochrome P450 MAX1 homologs catalyze distinct steps in strigolactone biosynthesis
Citation
Zhang, Y and van Dijk, ADJ and Scaffidi, A and Flematti, GR and Hofmann, M and Charnikhova, T and Verstappen, F and Hepworth, J and van der Krol, S and Leyser, O and Smith, SM and Zwanenburg, B and Al-Babili, S and Ruyter-Spira, C and Bouwmeester, HJ, Rice cytochrome P450 MAX1 homologs catalyze distinct steps in strigolactone biosynthesis, Nature Chemical Biology, 10, (12) pp. 1028-1033. ISSN 1552-4450 (2014) [Refereed Article]
Copyright Statement
Copyright 2014 Nature America
DOI: doi:10.1038/nchembio.1660
Abstract
Strigolactones (SLs) are a class of phytohormones and rhizosphere signaling compounds with high structural diversity. Three enzymes, carotenoid isomerase DWARF27 and carotenoid cleavage dioxygenases CCD7 and CCD8, were previously shown to convert all-trans-β-carotene to carlactone (CL), the SL precursor. However, how CL is metabolized to SLs has remained elusive. Here, by reconstituting the SL biosynthetic pathway in Nicotiana benthamiana, we show that a rice homolog of Arabidopsis MORE AXILLARY GROWTH 1 (MAX1), encodes a cytochrome P450 CYP711 subfamily member that acts as a CL oxidase to stereoselectively convert CL into ent-2’-epi-5-deoxystrigol (B-C lactone ring formation), the presumed precursor of rice SLs. A protein encoded by a second rice MAX1 homolog then catalyzes the conversion of ent-2’-epi-5-deoxystrigol to orobanchol. We therefore report that two members of CYP711 enzymes can catalyze two distinct steps in SL biosynthesis, identifying the first enzymes involved in B-C ring closure and a subsequent structural diversification step of SLs.
Item Details
Item Type: | Refereed Article |
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Keywords: | biosynthesis, catalyst, strigolactone |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the biological sciences |
UTAS Author: | Smith, SM (Professor Steven Smith) |
ID Code: | 101324 |
Year Published: | 2014 |
Web of Science® Times Cited: | 224 |
Deposited By: | Plant Science |
Deposited On: | 2015-06-17 |
Last Modified: | 2018-04-11 |
Downloads: | 0 |
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