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Rice cytochrome P450 MAX1 homologs catalyze distinct steps in strigolactone biosynthesis


Zhang, Y and van Dijk, ADJ and Scaffidi, A and Flematti, GR and Hofmann, M and Charnikhova, T and Verstappen, F and Hepworth, J and van der Krol, S and Leyser, O and Smith, SM and Zwanenburg, B and Al-Babili, S and Ruyter-Spira, C and Bouwmeester, HJ, Rice cytochrome P450 MAX1 homologs catalyze distinct steps in strigolactone biosynthesis, Nature Chemical Biology, 10, (12) pp. 1028-1033. ISSN 1552-4450 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 Nature America

DOI: doi:10.1038/nchembio.1660


Strigolactones (SLs) are a class of phytohormones and rhizosphere signaling compounds with high structural diversity. Three enzymes, carotenoid isomerase DWARF27 and carotenoid cleavage dioxygenases CCD7 and CCD8, were previously shown to convert all-trans-β-carotene to carlactone (CL), the SL precursor. However, how CL is metabolized to SLs has remained elusive. Here, by reconstituting the SL biosynthetic pathway in Nicotiana benthamiana, we show that a rice homolog of Arabidopsis MORE AXILLARY GROWTH 1 (MAX1), encodes a cytochrome P450 CYP711 subfamily member that acts as a CL oxidase to stereoselectively convert CL into ent-2-epi-5-deoxystrigol (B-C lactone ring formation), the presumed precursor of rice SLs. A protein encoded by a second rice MAX1 homolog then catalyzes the conversion of ent-2-epi-5-deoxystrigol to orobanchol. We therefore report that two members of CYP711 enzymes can catalyze two distinct steps in SL biosynthesis, identifying the first enzymes involved in B-C ring closure and a subsequent structural diversification step of SLs.

Item Details

Item Type:Refereed Article
Keywords:biosynthesis, catalyst, strigolactone
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Smith, SM (Professor Steven Smith)
ID Code:101324
Year Published:2014
Web of Science® Times Cited:224
Deposited By:Plant Science
Deposited On:2015-06-17
Last Modified:2018-04-11

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